CURRENT PERSPECTIVES OF DNA BARCODING IN BOSNIA AND HERZEGOVINA THROUGH BOLD DATABASE
DOI:
https://doi.org/10.54652/rsf.2020.v50.i1.40Keywords:
biodiversity databases, BOLD Systems, DNA barcodingAbstract
UDK: 577.212:574]:007.5(497.6)
DNA barcoding is a method designed to provide rapid and precise species identifications by using one or more of short gene sequences called barcodes. In most plant and fungi studies, the standard barcodes of choice are three plastid (rbcL, matK and trnH-psbA) and one nuclear (ITS) gene regions. The relatively high, but comparatively conserved rate of sequence evolution of mtDNA has made COI the marker of choice in animals. BOLD is a freely available cloud-based data storage and analysis platform developed with the aim to advance biodiversity science through DNA barcoding species identification. To date, over 6 million barcodes have been deposited in BOLD with 196,000 animal species, 68,000 plant species and 22,000 species of fungi and other organism entries. In this database, there are currently 447 entries for organisms from Bosnia and Herzegovina, which makes 0.0067% of the total number of BOLD entries. According to BOLD statistics, only 1.11% of all organism entries from B&H were submitted by B&H institutions.
Despite the fact that Bosnia and Herzegovina has valuable natural resources with a high percentage of endemic and autochthonous species, BOLD statistics elucidated the lack of coordinated and systematic DNA barcoding research so far. It is necessary to establish continuous progress of molecular-genetic characterization of these resources in the future. It is up to B&H institutions to decide if they want to continue the practice of submitting the data sporadically or if they will animate the research community to actively participate in this global project.
References
BOLD (2019). Barcode of life. (http://www.boldsystems.org/index.php) accessed: 03.02.2019.
Costa, F., Mamos T., Vieira, P.E. (2018). Aquatic macroinvertebrates diversity and evolution: some novel perspectives after 15 years of DNA barcoding [Abstract]. 3rd Central European Symposium for Macroinvertebrate Research. 19.
Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol., 3(5), 294-299.
Harms-Tuohy, C.A., Schizas, N.V., & Appeldoorn, R.S. (2016). Use of DNA metabarcoding for stomach content analysis in the invasive lionfish Pterois volitans in Puerto Rico. Mar. Ecol. Prog. Ser., 558, 181-191.
Hebert, P.D.N., Cywinska, A., Ball, S.L., & deWaard, J.R. (2003a). Biological identifications through DNA barcodes. Proc. R. Soc. Lond. B., 270, 313-321.
Hebert, P.D.N., Ratnasingham, S., deWaard, J.R. (2003b). Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc. R. Soc. Lond. B., 270, S96–S99.
iBOL (2017). What is DNA Barcoding? How DNA Barcoding Works and What it Will Do (http://www.ibol.org/phase1/about-us/what-is-dna-barcoding/) downloaded: 03.08.2018.
Kalamujić Stroil, B., Dorić, S., Hanjalić, J., Lasić, L., Pojskić, N. (2017). Regional biodiversity database (REBIDA) – The first comprehensive database of biological diversity of Bosnia and Herzegovina. Genetics & Applications, 1(2), 59-65.
Kalamujić Stroil, B., Dorić, S., Lukić Bilela, L., Pojskić, N. (2018b). Applied bioinformatics - Practicum. Sarajevo: Institute for Genetic Engineering and Biotechnology, Sarajevo.
Kalamujić Stroil, B., Lasić, L., Hanjalić, J., Mačar, S., & Vesnić, A. (2018a). The first DNA barcode record for Rhyacophila bosnica Schmid, 1970 and pairing of adult and larval life stages. Genetics & Applications, 2(2), 20-27.
Kress, W.J., & Erickson, D.L. (2008). DNA barcodes: Genes, genomics, and bioinformatics. PNAS. 105, 2761-2762.
Kress, W.J., & Erickson, D.L. (2012). DNA barcodes: Methods and protocols. Springer New York Dordrecht Heidelberg London: Springer Science+Business Media
Ratnasingham, S., & Hebert, P.D.N. (2007). BOLD: The Barcode of Life Data System. Mol. Ecol. Notes. 7(3), 355–364
Turnhout, E., & Boonman-Berson, S. (2011). Databases, scaling practices, and the globalization of biodiversity. Ecol. Soc., 16, 35.
