Porodica Fabaceae u polenskom spektru uzoraka meda iz Bosne i Hercegovine
DOI:
https://doi.org/10.54652/rsf.2025.v55.i2.624Ključne riječi:
Apiflora, Fabaceae, melisopalinologija, polen, med.Sažetak
Floristički sastav medonosne paše koji je određen specifičnom kombinacijom medonosnih biljaka na lokalitetu čini ključni faktor koji definiše fizičke, hemijske kao i biološke osobine meda. Melisopalinološki spektri predstavljaju vjeran odraz florističkog sastava medonosne paše, te je njihova analiza ključna u razumjevanju medonosnog potencijala medonosnog bilja. Cilj ovog istraživanja je na osnovu melisopalinološke analize uzoraka meda utvrditi: a) broj vrsta, b) procentualni udio i c) frekvencu pojavljivanja vrsta porodice Fabaceae u uzorcima meda iz Bosne i Hercegovine (BiH). U okviru istraživanja prikupljeno je i analizirano 100 uzoraka različitih tipova meda sa prostora Bosne i Hercegovine. Melisopalinološkom analizom je identifikovano 47 različitih tipova polena u ukupno 30 000 izbrojanih polenovih zrna. U botaničkom smislu, identifikovan je polen 24 porodica među kojima su najveći melisopalinološki značaj imala porodica Fabaceae, čija su polenova zrna konstatovana u čak 86% analiziranih profila. Na osnovu mikromorfoloških karaktera polenovih zrna identifikovane su sljedeće vrste/rodovi iz porodice Fabaceae: Robinia pseudoacacia L., Lotus corniculatus L., Trifolium pratense L., Trifolium repens L., Amorpha fruticosa L., Lathyrus sp., Medicago sativa L. i Onobrychis sp. Polenova zrna porodice Fabaceae su kao dominantna identifikovana u 22%, kao akcesorna u 40%, kao važna u 21% i kao minorna u 3% palinoloških profila. U okviru rada je prezentirano deset kategorija palinoloških profila koje su diferencirane na osnovu procentualnog udjela polenovih zrna porodice Fabaceae u pojedinačnim uzorcima. Specifično je da je od ukupnog broja uzoraka čak 70% imalo udio polenovih zrna ove porodice više od 10%. Na osnovu rezultata ovog istraživanja može se zaključiti da vrste iz porodice Fabaceae imaju veliki medonosni potencijal i predstavljaju važnu komponentu apiflore BiH. Rezultati istraživanja predstavljaju naučnu osnovu za unapređenje strategije održivog pčelarenja i očuvanje biodiverziteta autohtone apiflore BiH.
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References
Al-Kahtani, S.N., Abdou Taha, El-K., Al-Abdulsalam, M. (2017). Alfalfa (Medicago sativa L.) seed yield in relation to phosphorus fertilization and honeybee pollination. Saudi Journal of Biological Sciences, 24(5), 1051-1055.
Altay, V., Karahan, P., Karahan, F., Öztürk, M. (2018). Pollen analysis of honeys from Hatay/Turkey. Biological Diversity and Conservation, 11(3), 209-222.
Bahloul, R., Zerrouk, S., Chaibi, R. (2022). Pollen analysis of honey from Laghouat region (Algeria). Grana, 61(6), 471–480.
Balkanska, R., Stefanova, K., Stoikova-Grigorova, R., Gerginova, D., Simova, S., Atanassov, I. (2023). Assessment of the botanical origin of Bulgarian honey samples using melissopalynological, DNA barcoding and NMR analyses. Botanica Serbica, 47(1), 75-85.
Barbosaa, L.S.V., Galvãoa, C.R.S., Tchaickab, L., Silva-Almeidac, A.G., Barrosd. J.R.S. (2024). Pollen analysis of honey and pollen stored by Melipona (Melikerria) fasciculata Smith, 1854 (Apidae Meliponini), in an Amazon and Cerrado transition area, Maranhão, Brazil. Brazilian Journal of Biology, 84, e281066.
Boer, E. (2013). Risk assessment Robinia pseudoacacia L. Leiden: Naturalis Biodiversity Center.
Chiş, A. & Purcarea, C. (2011). Physico–Chemical Caracterisation of Artisanal Lime Honey from Bihor County. Natural resources and sustainable development, 1, 81-88.
DAISIE (2024). European Invasive Alien Species Gateway. http://www.europe-aliens.org/pdf/Robinia_pseudoacacia.pdf>
De Marco, A., Napoletano, P., Panico, S. C., Memoli, V., Santorufo, L., Ruggiero, A. G., Colombo C., Barile R., Maisto G. ( 2023). Combined effect of black locust invasion and fire on soils of Mediterranean shrublands and pine forests. Catena, 220A, 106656
Doan, L.P., Nguyen, T.T., Pham, M.Q., Tran, Q.T., Pham, Q.L., Tran, D.Q., Than, V.T., Bach, L.G. (2019). Extraction Process, Identification of Fatty Acids, Tocopherols, Sterols and Phenolic Constituents, and Antioxidant Evaluation of Seed Oils from Five Fabaceae Species. Processes, 7(7), 456.
Đogo Mračević, S., Krstić M., Lolić A., Ražić, S. (2020). Comparative study of the chemical composition and biological potential of honey from different regions of Serbia. Microchemical Journal, 152, 104420.
EPPO (2023). EPPO Global Database (available online). https://gd.eppo.int
Erdtman, G. (1943). An introduction to pollen analysis. Waltham, Mass., U.S.A.: Chronica Botanica Company.
Erdtman, G. (1952). Pollen morphology and plant taxonomy of angiosperms. Waltham, Mass., U.S.A.: Chronica Botanica Company.
Evelin, K., Raili, P., Kaie, M., Katrin. (2011). Physicochemical and melissopalynological characterization of Estonian summer honeys. Procedia Food Science, 1, 616-624
Gençay, Ç.Ö, Özenirler, Ç., Ecem B.N., Zare, G., Sorkun, K. (2018). Melissopalynological analysis for geographical marking of Kars honey. Kafas Universitesi eteriner altesi Dergisi, 24(1), 53-59.
Haedo, P.J., Martínez, C.L., Graffigna, S., Marrero, J.H., Torretta, P.J. (2022). Managed and wild bees contribute to alfalfa (Medicago sativa) pollination. Agriculture, Ecosystems & Environment, 324, 107711.
Haidamus, S.M., Affonso Lorenzon, M.C., Koshiyama, A.S., de Sousa Tassinari, W. (2019). Floral Diversity in Different Types of Honey. Brazilian Archives of Biology and Technology, 62, e19180241.
Harris, C., Ratnieks, F.L.W. (2024). Improving pollen and nectar supply by identifying the red clover (Trifolium pratense) cultivars that attract most pollinators. Agricultural and Forest Entomology, 26(2), 273-283.
Hederström, V., Johansson, S., Rundlöf, M., Svensson, P.G., Anderbrant, O., Lundin, O., Larsson, C.M., Lankinen, A. (2024). White clover pollinators and seed set in relation to local management and landscape context. Agriculture, Ecosystems & Environment, 365, 108933.
Hesse, M., Halbritter, H., Zetter, R., Weber, M., Büchner, R., Frosch-Radivo, A., Ulrich, S. (2009). Pollen Terminology An illustrated handbook. Wien, New York: Springer.
Hoag Lieux, M. (1972). A melissopalynological study of 54 Louisiana (U.S.A.) honeys. Review of Palaeobotany and Palynology, 13(2), 95-124.
Homrani, M., Escuredo, O., Rodríguez-Flores, M.S., Fatiha, D., Mohammed, B., Homrani, A., Seijo, M.C. (2020). Botanical Origin, Pollen Profile, and Physicochemical Properties of Algerian Honey from Different Bioclimatic Areas. Foods, 9, 938.
Hussein O., Seid A.(2024). Botanical origins of honeys from pollen analysis during the main honey flow across agro-ecologies in kelala district, South Wollo, Ethiopia. Heliyon, 10(21), e40101.
Ivanović, M., Galonja Coghill, T., Janković S., Rajković B.M., Stanković V., Paunović D., Zlatanović S. (2021). Analiza uzoraka organski proizvedenog meda i meda proizvedenog na konvencionalni način na području Zapadne Srbije. Zaštita materijala 62(1), 22-33.
Janarny, G., Ranaweera, K.K.D.S., Gunathilake, K.D.P.P. (2022). Digestive recovery of polyphenols, antioxidant activity, and anti-inflammatory activity of selected edible flowers from the family Fabaceae. Journal of food biochemistry, 46(2), e14052.
Khan, K., Malik, K., Ahmad, M., Qureshi, R., Asif Aziz, M., Gul, S., Al-Qahtani H. W., Khan, R. (2024). Diversity of melliferous Flora (Apiaries) in Honey and microscopic authentication using LM and SEM Techniques. Flora, 312, 152477.
Kolayli, S., Palabiyik, I., Atik, D.S., Keskin, M., Bozdeveci, A., Karaoglu, S.A. (2020). Comparison of Antibacterial and Antifungal Effects of Different Varieties of Honey and Propolis Samples. Acta Alimentaria, 49(4), 515-523.
Kozuharova, E., Benbassat, N. (2019). The Sainfoins (Onobrychis Mill., Fabaceae) – Forage For Grazing Animals, Honey And Medicinal Plants. EAS Journal of Pharmacy and Pharmacology, 1(2), 28-31.
Laxmikant, B., Devendra, M. (2014). Melittopalynological Investigation of winter honeys collected from Apis dorsata hives of Mul tahsil of Chandrapur District of Maharashtra State (India). International Refereed Journal of Engineering and Science, 2(3), 112-118.
Lazarević, B.K., Andrić, F., Trifković, J., Tešić, Ž., Milojković-Opsenica, D. (2012). Characterisation of Serbian unifloral honeys according to their physicochemical parameters. Food Chemistry 132(4), 2060–2064.
Lazzaro, L., Bolpagni, R., Buffa, G., Gentili, R., Lonati, M., Stinca, A., Rosario Acosta, A.T., Adorni, M., Aleffi, M., Allegrezza, M., Angiolini, C., Assini, S., Bagella, S., Bonari, G., Bovio, M., Bracco, F., Brundu, G., Caccianiga, M., Carnevali, L., Di Cecco, V., Ceschin, S., Ciaschetti, G., Cogoni, A., Foggi, B., Frattaroli, A.R., Genovesi, P., Gigante, D., Lucchese, F., Mainetti, A., Mariotti, M., Minissale, P., Paura, B., Pellizzari, M., Perrino, E.V., Pirone, G., Poggio, L., Poldini, L., Poponessi, S., Prisco, I., Prosser, F., Puglisi, M., Rosati, L., Selvaggi, A., Sottovia, L., Spampinato, G., Stanisci, A., Venanzoni, R., Viciani, D., Vidali, M., Villani, M., Lastrucci, L. (2020). Impact of invasive alien plants on native plant communities and Natura 2000 habitats: State of the art, gap analysis and perspectives in Italy. Journal of Environmental Management, 274, 111140.
Lazzaro, L., Mazza, G., D’Errico, G., Fabiani, A., Giuliani, C., Inghilesi, A. F., Lagomarsino, A., Landi, S., Lastrucci, L., Pastorelli, R., Roversi, PF., Torrini, G., Tricarico, E., Foggi. B. (2018). How ecosystems change following invasion by Robinia pseudoacacia: Insights from soil chemical properties and soil microbial, nematode, microarthropod and plant communities. Science of The Total Environment, 62, 1509–1518.
Louveaux, J., Maurizio, A., & Vorwohl, G. (1978). Methods of Melissopalynology. Bee World, 59, 139-157.
Luigi, S., Angelo, R., Adriano, S., Greta, L., Roberto, S., Sergio, R., Antonio, S. (2023). Wildfire promotes the invasion of Robinia pseudoacacia in the unmanaged Mediterranean Castanea sativa coppice forests. Frontiers in Forests and Global Change, 6, 1177551 .
Ljevnaić-Mašić, B., Nikolić, LJ., Džigurski, D., Ratkov, T., Popov, M., & Pihler, I. (2019). Medonosne biljke u kanalskoj mreži Banata. Acta Herbologica, 28(2), 133-144.
Majid, M., Ellulu, M. S., & Abu Bakar, M. F. (2020). Melissopalynological Study, Phenolic Compounds, and Antioxidant Properties of Heterotrigona itama Honey from Johor, Malaysia. Scientifica, 2020, 2529592.
Milojković Opsenica, D., Lušić, D., Tešić, Ž. (2015). Modern analytical techniques in the assessment of the authenticity of Serbian honey. Arhiv za higijenu rada i toksikologiju 66, 233-241.
Mišić, LJ. & Lakušić, R. (1990). Livadske biljke. Sarajevo: Svjetlost. Zavod za udžbenika i nastavna sredstva.
Murrellt, D.C., Shuelt R.W., Tomes D.T. (1982). Nectar production and floral characteristics in birdsfoot trefoil (Lotus corniculatus L.). Canadian journal of plant science, 62, 361-371.
Myrtsi, E.D., Evergetis, E., Koulocheri, S. D., Haroutounian, S.A. (2023). Bioactivity of Wild and Cultivated Legumes: Phytochemical Content and Antioxidant Properties. Antioxidants (Basel, Switzerland), 12(4), 852.
Nasir, H., Iqbal, Z., Hiradate, S., Fujii, Y. (2005). Allelopathic potential of Robinia pseudo-acacia L. Journal of Chemical Ecology 31(9), 2179-92
Nicolescu, V.N., Rédei, K., Mason, L.W., Torsten, Vor T., Pöetzelsberger, E., Bastien, J.C., Brus, R., Benča, T., Đodan, M., Cvjetkovic, B., Andrašev, S., Porta, L.N., Lavnyy, V., Mandžukovski, K. P., Roženbergar, D., Wąsik, R., Godefridus, M.J.M., Monteverdi M.C., Musch, B., Klisz, M., Perić, S., Keça, LJ., Bartlett, D., Hernea C., Pástor, M. (2020) Ecology, growth and management of black locust (Robinia pseudoacacia L.), a non-native species integrated into European forests. Journal of Forestry Research Article, 31, 1081–1101.
Oberdörfer, E. (1957). Pflanzensoziologische Exkursions Flora. Sttutgart: Eugen Ulmer Verlag.
Ozenirler, C., Gencay C.O., Ecem B.N., Dikmen, F., Zare, G., Celikbicak, O., Sorkun, K. (2019). Physicochemical and Palynological Characterization of the Onobrychis Miller (Fabaceae) Honey. Gazi University Journal of Science, 32(1), 63-76.
Pătruică, S., Alexa, E., Obiștioiu, D., Cocan, I., Radulov, I., Berbecea, A., Lazăr, R.N., Simiz, E., Vicar, N.M., Hulea, A., Moraru, D. (2022). Chemical Composition, Antioxidant and Antimicrobial Activity of Some Types of Honey from Banat Region, Romania. Molecules, 27, 4179.
Pellissier, V., Muratet A., Verfaillie, F., Machon, N. (2012). Pollination success of Lotus corniculatus (L.) in an urban context. Acta Oecologica, 39, 94-100.
Persano Oddo, L., Piro, R., Bruneau, E., Guyot-Declerck, C. Ivanov, T., Piškulová, J., Flamini, C., Lheritier, J., Morlot, M., Russmann, H., Von Der Ohe, W., Von Der Ohe, K., Gotsiou, P., Karabournioti, S., Kefalas, P., Passaloglou-Katrali, M., Thrasyvoulou, A., Tsigouri, A., Marcazzan, G. L., Piana, M. L., Piazza, M. G., Sabatini, A. G., Kerkvliet, J., Godinho, J., Bentabol, A., Ortiz Valbuena, A., Bogdanov, S., Ruoff, K. (2004): Main European unifloral honeys: descriptive sheets. Apidologie, 35, 38–S81.
Rašić, S., Štefanić, E., Antunović, S., Jović, J., Kristek, S. (2018). Peludna analiza meda sjeveroistočne Hrvatske. Poljoprivreda, 24(2), 43-49.
Raweh, H.S.A., Badjah-Hadj-Ahmed, A.Y., Iqbal, J., Alqarni, A.S. (2023). Physicochemical Composition of Local and Imported Honeys Associated with Quality Standards. Foods (Basel, Switzerland), 12(11), 2181.
Redžić, S., Barudanović, S., Radević, M. (2008). Bosna i Hercegovina – zemlja raznolikosti. Sarajevo: Federalno ministarstvo okoliša i turizma.
Richardson, DM, Rejmánek, M. (2011).Trees and shrubs as invasive alien species – a global review. Diversity Distributions, 17, 788–809.
Shakoori, Z., Salaseh, E., Mehrabian, A. R., Tehrani, D. M., Dardashti, N. F., & Salmanpour, F. (2024). The amount of antioxidants in honey has a strong relationship with the plants selected by honey bees. Scientific reports, 14(1), 351.
Shuxuan, J., Per, K., Bo, M., Birte, B. (2021). Pollination and Plant Reproductive Success of Two Ploidy Levels in Red Clover (Trifolium pratense L.). Frontiers in Plant Science, 12, 720069.
Simanonok, M.P., Otto, C.R.V., Iwanowicz D.D., Cornman, R. S. (2021). Honey bee-collected pollen richness and protein content across an agricultural land-use gradient. Apidologie 52, 1291–1304.
Official Gazette of B&H (2009). Rulebook on methods for the control of honey and other bee products of Bosnia and Herzegovina. 37/09. (in Bosnian)
Sodré, G., da S., Marchini, L. C., Carvalho, C. A., & Moreti, A. C. (2007). Pollen analysis in honey samples from the two main producing regions in the Brazilian northeast. Anais da Academia Brasileira de Ciencias, 79(3), 381–388.
Song, X-Y, Yao, Y-F, Yang, W-D (2012). Pollen Analysis of Natural Honeys from the Central Region of Shanxi, North China. PLoS ONE 7(11), e49545.
Stanimirović, Z., Soldatović, B., Vučinić, M. (2000). Biologija pčela. Medonosna pčela. Beograd: Medicinska knjiga.
Stevanović B., Janković M. (2001). Ekologija biljaka sa osnovama fiziološke ekologije biljaka. Beograd: NNK International.
Šarić, G., Matković, D., Hruškar, M., Vahčić, N. (2008). Characterisation and Classification of Croatian Honey by Physicochemical Parameters. Food Technology and Biotechnology 46(4), 355–367.
Tomczyk, M., Tarapatskyy, M., & Džugan, M. (2019). The influence of geographical origin on honey composition studied by Polish and Slovak honeys. Czech Journal of Food Sciences, 37(4), 232–238
Topal, A., Uzun, A., Polat, O. (2023). Apifloristic diversity in the eastern Mediterranean region: implications for biodiversity conservation and use. Archives of Biological Sciences, 75(2), 221-236.
Tulu, D., Aleme, M., Mengistu, G., Bogale A., Bezabeh, A., Mendesil, E. (2023). Melissopalynological analysis and floral spectra of Apis mellifera scutellata Lepeletier bees in different agroecologies of southwest Ethiopia. Heliyon, 9(5), e16047.
Tungmunnithum, D., Drouet, S., Lorenzo, J. M., & Hano, C. (2021). Characterization of Bioactive Phenolics and Antioxidant Capacity of Edible Bean Extracts of 50 Fabaceae Populations Grown in Thailand. Foods, 10(12), 3118.
Umeljić, V. (2013). Atlas medonosnog bilja 2. Kragujevac.
Umeljić, V. (2015). Atlas medonosnog bilja 1. Kragujevac.
Uršulin-Trstenjak, N., Levanić, D., Galić, A., Barušić, L., Jurica, K., Vahčić, N. (2014). Confirming the botanical origin of the Croatian black locust honey (Istria region) using physicochemical parameters during two seasons. Journal of Hygienic Engineering and Design, 8, 124-128.
Uršulin-Trstenjak, N., Levanić, D., Šušnić, S., Lasić, D, Šušnić, V. (2015). Determination of geographical origin of black locust honey of five Croatian regions by applying the PCA method. Journal of Hygienic Engineering and Design, 13, 68-74.
Uršulin-Trstenjak, N., Puntarić, D., Levanić, D., Gvozdić, V., Pavlek, Ž., Puntarić, A., Puntarić, E., Puntarić, I., Vidosavljević, D., Lasić, D., Vidosavljević, M. (2017). Pollen, Physicochemical, and Mineral Analysis of Croatian Acacia Honey Samples: Applicability for Identification of Botanical and Geographical Origin. Journal of Food Quality, 2017,(1), 8538693.
Villalpando-Aguilar, J.L., Quej-Chi, V.H., López-Rosas, I., Cetzal-Ix, W., Aquino-Luna, V.Á., Alatorre-Cobos, F., Martínez-Puc, J.F. (2022). Pollen Types Reveal Floral Diversity in Natural Honeys from Campeche, Mexico. Diversity, 14(9), 740.
Vítková, M., Müllerová, J., Sádlo, J., Pergl, J., Pyšek, P. (2017). Black locust (Robinia pseudoacacia) beloved and despised: A story of an invasive tree in Central Europe. Forest Ecology and Management, 384, 287-302.
Von Der Ohe, W., Persano Oddo, L., Piana, M. L., Morlot, M., Martin, P. (2004). Harmonized methods of melissopalynology. Apidologie, 35, 18-25.
Youssef, A. M. M., Maaty, D. A. M., & Al-Saraireh, Y. M. (2023). Phytochemical Analysis and Profiling of Antioxidants and Anticancer Compounds from Tephrosia purpurea (L.) subsp. apollinea Family Fabaceae. Molecules, 28(9), 3939.
Zhu, M., Zhao, H., Wang, Q., Wu, F., & Cao, W. (2020). A Novel Chinese Honey from Amorpha fruticosa L.: Nutritional Composition and Antioxidant Capacity In Vitro. Molecules, 25(21), 5211.

