Određivanje antioksidacijske aktivnosti tri vrste familije Lamiaceae primjenom Briggs-Rauscher reakcione metode
DOI:
https://doi.org/10.54652/rsf.2017.v47.i1.68Ključne riječi:
Briggs-Rauscher reaction, Lamiaceae plants, inhibition, antioxidantSažetak
UDK: 582.929.4:581.19]:544
Antioksidacijska aktivnost vodenih i vodeno-etanolnih ekstrakata iz lišća matičnjaka, ruzmarina i kadulje ispitivana je primjenom Briggs-Rauscher reakcione metode. Ova metoda se bazira na inhibitorskom efektu antioksidanasa na oscilacije Briggs-Rauscher reakcione smjese. Efekat se sastoji u trenutačnom zaustavljanju oscilacija, vremenu inhibicije i ponovnoj regeneraciji oscilacija. Vrijeme inhibicije linearno zavisi od koncentracije i vrste dodanog antioksidansa. Sa porastom koncentracije ekstrakata linearno se povećavalo vrijeme inhibicije. Vodeni ekstrakti kadulje dobijeni na temperaturi ključanja uz refluks su pokazali najbolju sposobnost inhibicije oscilacija tj., najveću antioksidacijsku aktivnost. Antioksidacijska aktivnost ekstrakata je opadala prema nizu kadulja > matičnjak > ruzmarin nezavisno od načina ekstrakcije. Također je ispitana i antioksidacijska aktivnost šest dvokomponentnih smjesa vodenih ekstrakata dobijenih na temperaturi ključanja uz refluks.
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