Određivanje ukupnih fenola i antioksidacijske aktivnosti vodenih ekstrakata odabranih ljekovitih biljaka
DOI:
https://doi.org/10.54652/rsf.2017.v47.i2.61Ključne riječi:
plant extract, total phenolic content, antioxidant activity, Briggs-Rauscher reactionSažetak
UDK: 633.88:547.56
Cilj ovog rada je da se analizira i usporedi sadržaj ukupnih fenola i antioksidacijske aktivnosti vodenih ekstrakata tri ljekovite biljke: hibiskusa (Hibiscus spp.), ruzmarina (Rosmarinus spp.) i vrijeska (Satureja spp.). Biljni ekstrakti pripremljeni su postupkom maceracije. Sadržaj ukupnih fenola određivan je spektrofotometrijski Folin-Ciocalteu metodom koristeći galnu kiselinu kao standard. Antioksidacijska aktivnost ispitivana je primjenom oscilirajuće Briggs-Rauscher reakcije. Vrijeme inhibicije oscilacija Briggs-Rauscher reakcione smjese uzrokovano biljnim ekstraktima je praćeno potenciometrijskom metodom. Sadržaj ukupnih fenola kretao se od 0.27 mgGAE/g suhog ekstrakta za hibiskus, 1.29 mgGAE/g suhog ekstrakta za ruzmarin do 1.43 mgGAE/g suhog ekstrakta za vrijesak. Antioksidacijska aktivnost ekstrakata je opadala prema nizu: vrijesak > ruzmarin > hibiskus. Linearnom regresijom utvrđena je visoka korelacija između sadržaja ukupnih fenola i vremena inhibicije Briggs-Rauscher reakcije (R2 = 0.994), dok je između sadržaja ukupnih fenola i relativnog Briggs-Rauscher antioksidacijskog indeksa korelacija bila niža (R2 = 0.814).
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