DETERMINATION OF VITAMIN C IN SOME BOSNIAN CRATAEGUS L. SPECIES BY SPETROPHOTOMETRIC METHOD

Azra Tahirović; Amira  Čopra – Janićijević; Neđad Bašić; Danijela  Vidić; Lejla  Klepo; Delila  Delić

doi:10.54652/rsf.2012.v42.i1.129

Autor(i)

Azra Tahirović Faculty of Forestry University of Sarajevo
Amira Čopra – Janićijević Faculty of Sciences University of Sarajevo
Neđad Bašić Faculty of Forestry University of Sarajevo
Danijela Vidić Faculty of Sciences University of Sarajevo
Lejla Klepo Faculty of Sciences University of Sarajevo
Delila Delić

DOI:

https://doi.org/10.54652/rsf.2012.v42.i1.129

Ključne riječi:

ascorbic acid, Crataegus, hawthorns, methylene blue, spectrophotometry

Sažetak

UDK 577.164.2:582.711.714

535.234:577.164.2

U ovoj studiji su prikazani rezultati određivanja vitamina C u plodovima odabranih bosanskih vrsta glogova (Crataegus L). Određivanje vitamina C je vršeno primjenom spektrofotometrijske metode, zasnovane na kinetičkoj reakciji askorbinske kiseline (AA) sa metilenskim plavim (MB⁺). Mjerenja intenziteta apsorpcije su vršena na apsorpcionom maksimumu, λ_max = 665 nm. U studiji je korištena i poredbena titracijska metoda analize sadržaja vitamina C u cilju komparacije i evaluacije dobivenih rezultata. Sadržaj vitamina C u ispitivanim uzorcima plodova različitih vrsta glogova se kretao u rasponu 106,12 – 231,96 mg vitamina C na 100 g suhog uzorka ploda. Dobivene vrijednosti iskorištenja (recovery) za spektofotometrijsku metodu, su se kretale od 101,4% do 108,0%, dok je vrijednost relativne standardne devijacije (RSD) iznosila 0,40 % - 3,37 %. Iz dobivenih rezultata određivanja sadržaja vitamina C u ispitivanim uzorcima plodova odabranih vrsta glogova može se uočiti da su sadržaji vitamina C u svim ispitavanim uzorcima znatno visoki. Vrste C. rhipidophylla, C. microphylla i C.× subsphaerice ,koje nisu uvrštene u oficijelnu Evropsku farmakopeju, imaju čak i veće vrijednosti vitamina C u odnosu na priznatu ljekovitu vrstu, C. monogyna. Najveći sadržaj vitamina C je određen u plodu endemične vrste, C. microphylla i u odnosu na druge ispitivane vrste je veći za približno dva puta.

Dobiveni rezultati jasno ukazuju da su plodovi ispitivanih vrsta glogova dobar izvor vitamina C kao prirodnog antioksidanta.

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