In vitro antioxidant activity of methanolic extracts of the different types of commercial pepper
DOI:
https://doi.org/10.54652/rsf.2022.v52.i2.379Abstract
Pepper is one of the most famous and widespread spices in the world. This commercial plant belongs to the Piperaceae family. Depending on the treatment to which the pepper fruit is exposed, we distinguish between black, white and green pepper. In this research, methanolic extracts of different types of pepper (Piper nigrum) and pink pepper (Schinus terebinthifolius Raddi) were prepared. Maceration and ultrasonic extraction were used to extract bioactive components from pepper samples. The reduction potential of the extracts was tested using the FRAP method. The efficiency of free radical inhibition was determined by the DPPH method. Pink pepper extract showed the highest antioxidant activity in in vitro conditions. High antioxidant activity was also recorded in green pepper extracts. In general, the extracts showed high potency in neutralizing free radicals.
References
Ahmad, N., Fazal, H., Abbasi, B.H., Farooq, S., Ali, M., Khan, M.A. (2012). Biological role of Piper nigrum L. (Black pepper): A review. Asian Pacific Journal of Tropical Biomedicine, 2(3), S1945-S1953.
Benzie, I.F.F., Strain, J.J. (1999). Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299, 15-27.
Dayem, A.A., Choi, H.Y., Yang, G.M., Kim, K., Saha, S.K., Cho, S.G. (2016). The anti-cancer effect of polyphenols against breast cancer and cancer stem cells: molecular mechanisms. Nutrients. 8(9), 581.
Dinesha, R., Chikkanna, D. (2014). Antioxidant activities of Pippali (Piper longum) proteins. International Journal of Pharmaceutics and Drug Analysis, 2(11), 811-814.
Horozić, E. Zukić, A., Kolarević, L., Bjelošević, D., Ademović, Z., Šarić-Kundalić, B., Husejnagić, D., Kudumović, A., Hamzić, S. (2019). Evaluation of antibacterial and antioxidant activity of methanol needle extracts of Larix Decidua Mill., Picea Abies (L.) H. Karst. and Pinus Nigra J. F. Arnold. Technics Technologies Education Management, 14(1), 14-19.
Hritcu, L., Noumedem, J.A., Cioanca, O., Hancianu, M., Postu, P., Mihasan, M. (2015). Anxiolytic and antidepressant profile of the methanolic extract of Piper nigrum fruits in beta-amyloid (1–42) rat model of Alzheimer’s disease. Behavioral and Brain Functions, 11, 13.
Karsha, P.V., Lakshmi, O.B. (2010). Antibacterial activity of black pepper (Piper nigrum Linn.) with special reference to its mode of action of bacteria. Indian Journal of Natural Products and Resources, 1(2), 213-215.
Kavitha, S., Mani, P. (2017). Anti-bacterial Activity of Extract of Piper nigrum Leaf. BioTechnology: An Indian Journal, 13(4), 144.
Khajuria, A., Thusu, N., Zutshi, U. (2002). Piperine modulates permeability characteristics of intestine by inducing alterations in membrane dynamics: Influence on brush border membrane fluidity, ultra-structure and enzyme kinetics. Phytomedicine, 9(3), 224-231.
Khan, A.U., Talucder, M.S.A., Das, M., Norees, S., Pane, Y.S. (2021). Prospect of The Black Pepper (Piper nigrum L.) as Natural Product Used to an Herbal Medicine. Open Access Macedonian Journal of Medical Sciences, 9(F), 563-573.
Khom, S., Strommer, B., Schöffmann, A., Hintersteiner, J., Baburin, I., Erker, T., Schwarz, T., Schwarzer, C., Zaugg, J., Hamburger, M., Hering, S. (2013). GABAA receptor modulation by piperine and a non-TRPV1 activating derivative. Biochemical Pharmacology, 85(12), 1827-1836.
Kim, D.W., Kim, M.J., Shin, Y., Jung, S.K., Kim, Y.J. (2020). Green Pepper (Piper nigrum L.) Extract Suppresses Oxidative Stress and LPS-Induced Inflammation via Regulation of JNK Signaling Pathways. Applied Sciences, 10, 2519.
Mathew, P.J., Mathew, P.M., Kumar, V. (2001). Graph clustering of Piper nigrum L. (black pepper). Euphytica, 118(3), 257-264.
Merlo, T.C., Contreras-Castillo, C.J., Saldaña, E., Barancelli, G.V., Dargelio, M.D.B., Yoshida, C.M.P., Ribeiro Junior, E.E., Massarioli, A., Venturini, A.C. (2019). Incorporation of pink pepper residue extract into chitosan film combined with a modified atmosphere packaging: Effects on the shelf life of salmon fillets. Food Research International, 125, 108633.
Menegali, B.S., Selani, M.M., Saldaña, E., Patinho, I., Diniz, J.P., Melo, P.S., Filho, N.J.P., Contreras-Castillo, C.J. (2020). Pink pepper extract as a natural antioxidant in chicken burger: Effects on oxidative stability and dynamic sensory profile using Temporal Dominance of Sensations. LWT, 121, 108986.
Paarakh, P.M., Sreeram, D.C., Shruthi, S.D., Ganapathy, S.P.S. (2015). In vitro cytotoxic and in silico activity of piperine isolated from Piper nigrum fruits Linn. In Silico Pharmacology, 3, 9.
Pradhan, K.J., Variyar, P.S., Bandekar, J.R. (1999). Antimicrobial activity of novel phenolic compounds from green pepper (Piper nigrum L.). LWT - Food Science and Technology, 32(2), 121-123.
Rani, S.K.S., Saxena, N. (2013). Antimicrobial activity of black pepper (Piper nigrum L.). Global Journal of Pharmacology, 7(1), 87-90.
Reddy, M.N., Reddy, N.R., Jamil, K. (2015). Spicy anti-cancer spices: A review. International Journal of Pharmacy and Pharmaceuticals Sciences, 7(11), 1-6.
Romani, V.P., Hermández, C.P., Martins, V.G. (2018). Pink pepper phenolic compounds incorporation in starch/protein blends and its potential to inhibit apple browning. Food Packaging and Shelf Life, 15, 151-158.
Serrano-León, J.S., Bergamaschi, K.B., Yoshida, M.P., Saldaña, E., Selani, M.M., Rios-Mera, J.D., Alencar, S.M., Contreras-Castillo, C.J. (2018). Chitosan active films containing agro-industrial residue extracts for shelf life extension of chicken restructured product. Food Research International, 108, 93-100.
Singleton, V.L., Orthofer, R., Lamuela-Raventós, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 299, 152-178.
Srinivasan, K. (2007). Black pepper and its pungent principle-piperine: A review of diverse physiological effects. Critical Reviews in Food Science and Nutrition, 47(8), 735-748.
Sruthi, D., John Zachariah T. (2017). In vitro antioxidant activity and cytotoxicity of sequential extracts from selected black pepper (Piper nigrum L.) varieties and Piper species. International Food Research Journal, 24(1), 75-85.
Tiwari, P., Singh, D., Shing, M.M. (2008). Anti-Trichomonas activity of Sapindus saponins, a candidate for development as microbicidal contraceptive. Journal of Antimicrobial Chemotherapy, 62(3), 526-534.
Tripathi, A.K., Jain, D.C., Kumar, S. (1996). Secondary metabolites and their biological and medical activities of Piper species plants. Journal of Medicinal and Aromatic Plant Studies, 18, 302-321.
Zahin, M., Bokhari, N.A., Ahmad, I., Husain, F.M., Althubiani, A.S., Alruways, M.W., Perveen, K., Shawali, M. (2021). Antioxidant, antibacterial, and antimutagenic activity of Piper nigrum seeds extracts. Saudi Journal of Biological Sciences, 28(9), 5094-5105.
Zarai, Z., Boujelbene, E., Ben Salem, N., Gargouri, Y., Sayari, A. (2013). Antioxidant and antimicrobial activities of various solvent extracts, piperine and piperic acid from Piper nigrum. LWT Food Science and Technology, 50(5), 634-641.
Zaugg, J., Baburin, I., Strommer, B., Kim, H.J., Hering, S., Hamburger, M. (2010). HPLC-based activity profiling: Discovery of piperine as a positive GABAA receptor modulator targeting a benzodiazepine-independent binding site. Journal of Natural Products, 73(2), 185-191.
Zhang, C., Zhao, J., Famous, E., Pan, S., Peng, X., Tian, J. (2021). Antioxidant, hepatoprotective and antifungal activities of black pepper (Piper nigrum L.) essential oil. Food Chemistry, 346, 128845.
Zhang, J., Ye, K.P., Zhang, X., Pan, D.D., Sun, Y.Y., Cao, Y.X. (2017). Antibacterial activity and mechanism of action of black pepper essential oil on meat-borne Escherichia coli. Frontiers in Microbiology, 7, 2094.
Zhaomei, M., Hachem, P., Hensley, H., Stoyanova, R., Kwon, H.W., Hanlon, A.L., Agrawal, S., Pollack, A. (2008). Antisense MDM2 Enhances the response of androgen insensitive human prostate cancer cells to androgen deprivation in vitro and in vivo. Prostate, 68(6), 599-609.
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Copyright (c) 2022 Emir Horozić, Dženita Sinanović, Sabahudin Halilović, Irma Džafić, Semiha Bajrić
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