Utjecaj omjera vode i organskih otapala na antioksidativno djelovanje i sadržaj bioaktivnih komponenti u ekstraktima Artemisia annua L. i Artemisia absinthium L.
DOI:
https://doi.org/10.54652/rsf.2024.v54.i2.594Ključne riječi:
Wormwood, Polyphenols, Flavonoids, FRAP, DPPH inhibition.Sažetak
Gorki (Artemisia absinthium L.) i slatki pelin (A. annua L.) tradicionalne su biljne vrste u Asteraceae (Compositae). Njihova upotreba u tradicionalnoj medicini odavno je poznata jer se čajevi i tinkture spravljeni od njih koriste u liječenju bolesti probavnog sistema. U radu je ispitan sadržaj bioaktivnih komponenti (polifenola i flavonoida) i antioksidativno djelovanje (FRAP i DPPH metodom) navedenih vrsta. Za analizirane vrste pripremljena je serija ekstrakata sa odabranim rastvaračima (voda, metanol, etanol i aceton) te smjesama navedenih organskih rastarača sa vodom u različitim volumnim omjerima. Rezultati istraživanja su pokazali da ekstrakti slatkog pelina imaju više bioaktivnih komponenti a time i izraženije antioksidativno djelovanje u odnosu na ekstrakte gorkog pelina. U smislu učinkovitosti ekstrakcije, najučinkovitijom se pokazala mješavina acetona i vode (20:30 v/v). Što se tiče čistih organskih otapala, najučinkovitija za izolaciju bioaktivnih komponenti je voda, dok je aceton pokazao najslabiju ekstrakcijsku moć.
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