Doprinos molekularno genetičkoj karakterizaciji Helleborus multifidus Vis. u Bosni i Hercegovini
DOI:
https://doi.org/10.54652/rsf.2016.v46.i2.71Ključne riječi:
Helleborus multifidus, trnL, matK, ITS1, ITS2Sažetak
UDK: 582.675.1:575(497.6)
H. multifidus je endemična ilirsko-jadranska vrsta rasprostranjena u Italiji, Sloveniji, Hrvatskoj, Bosni i Hercegovini, Crnoj Gori i Albaniji. Iako nekoliko studija navodi različite taksonomske kategorije za H. multifidus, prvi put su korišteni molekularno-genetički markeri (trnL region hloroplastne DNA, matK, ITS 1 i ITS 2) u genetičkoj karakterizaciji populacija H. multifidus sa tri lokaliteta u Bosni i Hercegovini. Rezultati provedenog istraživanja su pokazali da PCR-RFLP metoda trnL introna nije informativna u utvrđivanju interpopulacijskog i intrapopulacijskog diverziteta analiziranog taksona. Međutim, sekvence matK, ITS1 i ITS2 ukazuju na postojanje razlika između populacija kukurjeka iz okoline Trebinja i populacija sa Kupreškog polja, ukazujući na nužnost dodatnih analiza.
References
BENSON, D.A., KARSCH-MIZRACHI, I., LIPMAN, D.J., OSTELL, J. & WHEELER, D.L. (2003): GenBank. Nucleic Acids Res., 31, 23–27.
BRODY, J.R., KERN, S.E. (2005): Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis. Biotechniques, 38(1), 60.
BURLAND, T.G. (2000): DNASTAR's Lasergene sequence analysis software. Methods Mol Biol., 132, 71-91.
FIOR, S., KARIS, P.O., CASAZZA, G., MINUTO, L., SALA, F. (2006): Molecular phylogeny of the Caryophyllaceae (Caryophyllales) inferred from chloroplast matK and nuclear rDNA ITS sequences. Am J Bot, 93(3), 399-411.
HALL, T. A. (1999): BIOEDIT: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser, 41, 95–98.
MARTINIS, Z. (1973): Genus: Helleborus L. U: Martinis Z., Regula-Berilacqua Lj., Trinajstić I. (Eds.) (1973): Genus: Helleborus. Suppl. Fl. Anal. Jugosl., 1(2): 235-240.
MATHEW, B. (1989): Hellebores. Alpine Garden Society, St. John's Woking, Surrey, UK.
MEINERS, J., DEBENER, T., SCHWEIZER, G., WINKELMANN, T. (2011): Analyisis of the taxonomic subdivision within the genus Helleborus by nuclear DNA content and genome-wide DNA markers. Sci. Hort., 128: 38-47.
PADMALATHA, K., JAYARAM, K., PRASAD, M.N.V. (2008): A Rapid Protocol for the Isolation of Polysaccharide- and Polyphenolic-Free Genomic DNA for RAPD Analysis of Threatened Medicinal Plants. Med. Aromat. Plant Sci. Biotechnol., 2, 50-53.
PEARSON, W.R. (1994): Using the FASTA program to search protein and DNA sequence databases. Methods Mol Biol, 24, 307-31.
ROSKOV Y., ABUCAY L., ORRELL T., NICOLSON D., BAILLY N., KIRK P.M., BOURGOIN T., DEWALT R.E., DECOCK W., DE WEVER A., NIEUKERKEN E. VAN, ZARUCCHI J., PENEV L., eds. (2017). Species 2000 & ITIS Catalogue of Life, 22nd March 2017. Digital resource at www.catalogueoflife.org/col. Species 2000: Naturalis, Leiden, the Netherlands. ISSN 2405-8858
SUN, H., MCLEWIN, W., FAY, M.F. (2001): Molecular phylogeny of Helleborus (Ranunculaceae), with an emphasis on the East Asian-Mediterranean disjunction. Taxon, 50, 1001-1018.
ŠILIĆ, Č. (1990): Endemične biljke. Svjetlost, Sarajevo. pp. 36.
TABERLET, P., GIELLY, L., PAUTOU, G., BOUVET, J. (1991): Universal primers for amplification of three noncoding regions of chloroplast DNA. Plant Mol. Biol., 17, 1105-1109.
THOMPSON, J. D., HIGGINS, D.G., GIBSON, T. J. (2011): CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22(22), 4673–4680.
TUTIN, T.G. (1996): Genus Helleborus L. In: Tutin T.G., Burges N.A, Chater A.O., Edmondson J.R., Heywood V.H., Moore D.M., Valentine D.H., Walters S.M., Webb D.A. (Eds.) (1996): Flora Europaea, vol 1. Cambridge: Cambridge Univ. Press, pp. 249-251.
WATERHOUSE, A.M., PROCTER, J.B., MARTIN, D.M.A., CLAMP, M., BARTON, G.J. (2009): Jalview Version 2-a multiple sequence alignment editor and analysis workbench. Bioinformatics, 25, 1189-1191.
WHITE, T.J., BRIMS, T., LEE, S., TAYLOR, J. (1990): Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In „PCR Protocols: A Guide to Methods and Applications“ (M. Innis, D. Gelfand, J. Sninsky, and T. White, Eds.), pp. 315-322, San Diego: Academic Press.
ZONNEVELD, B.J.M. (2001): Nuclear DNA contents of all species of Helleborus (Ranunculaceae) discriminate between species and sectional division. Plant Syst. Evol., 229: 125-130.
