CONTRIBUTION TO MOLECULAR GENETIC CHARACTERIZATION OF HELLEBORUS MULTIFIDUS Vis. IN BOSNIA AND HERZEGOVINA
DOI:
https://doi.org/10.54652/rsf.2016.v46.i2.71Keywords:
Helleborus multifidus, trnL, matK, ITS1, ITS2Abstract
UDK: 582.675.1:575(497.6)
Helleborus multifidus Vis. is endemic Illyric-Adriatic species with distribution range in Italy, Slovenia, Croatia, Bosnia and Herzegovina, Montenegro and Albania. Although few studies reported different taxonomic categories for H. multifidus, this one is the first using molecular-genetic markers (trnL region and matK of chloroplast DNA and nuclear ITS1 and ITS2 region) for genetic characterization of H. multifidus presented at three localites in Bosnia and Herzegovina. The results revealed that PCR-RFLP on trnL intron was not informative for testing inter- or intrapopulation diversity. Contrary, analysis of matK, ITS1 and ITS2 sequences showed differences between populations from Trebinje region and Kupreško polje, pointing to the need to include additional analyses in order to confirm these findings.
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