Određivanje vitamina C u cvjetovima nekih bosanskohercegovačkih vrsta roda Crataegus L.

Autor(i)

  • Azra Tahirović Faculty of Forestry University of Sarajevo
  • Amira Čopra – Janićijević Faculty of Sciences University of Sarajevo
  • Neđad Bašić Faculty of Forestry University of Sarajevo
  • Lejla Klepo Faculty of Sciences University of Sarajevo
  • Mirel Subašić Faculty of Forestry University of Sarajevo

DOI:

https://doi.org/10.54652/rsf.2012.v42.i2.119

Ključne riječi:

ascorbic acid, Crataegus, hawthorns, flowers, spectrophotometry, Vitamin C

Sažetak

UDK 582.711.714:577.164.2

          543.42:577.164.2

U studiji su prikazani rezultati određivanja vitamina C u cvjetovima odabranih bosanskohercegovačkih vrsta glogova (Crataegus L). U ispitivanjima korištene su tri vrste i dva hibrida glogova i to: C. monogyna, C. rhipidophylla, C. microphylla, C.× macrocarpa i C.× subsphaerice.

Određivanje vitamina C u biljnim uzorcima je vršeno primjenom spektrofotometrijske metode, zasnovane na kinetičkoj reakciji askorbinske kiseline (AA) sa metilenskim plavim (MB+). Mjerenja intenziteta apsorpcije su vršena na apsorpcionom maksimumu, λmax = 665 nm. U studiji je korištena i poredbena titrimetrijska metoda analize sadržaja vitamina C zasnovana na jodimetrijskom određivanju uz indikator skrob.

Sadržaj vitamina C u ispitivanim uzorcima cvjetova različitih vrsta glogova se kretao u rasponu 617,07 – 1104 mg vitamina C na 100 g suhog uzorka cvijeta. Dobivene vrijednosti iskorištenja (recovery) za spektofotometrijsku metodu, su se kretale od 94% do 100,0%, dok je vrijednost relativne standardne devijacije (RSD) iznosila 4,5 % - 6,7 % u odnosu na referentnu metodu.

Iz dobivenih rezultata određivanja sadržaja vitamina C u ispitivanim uzorcima cvjetova odabranih vrsta glogova može se uočiti da su sadržaji vitamina C u svim ispitavanim uzorcima znatno visoki. Vrste C. rhipidophylla, C. monogyna kao i njihov hybrid C.× subsphaericea imaju dosta slične vrijednosti sadržaja vitamina C i veći u odnosu na druge ispitivane specije. C. macrocarpa po vrijednosti sadržaja vitamina C je dosta slična vrsti C. rhipidophylla. Najmanji sadržaj vitamina C je određen u cvijetu endemične vrste, C. microphylla i u odnosu na oficijelnu ljekovitu vrstu, C. monogyna, manji je za približno dva puta.

U odnosu na prethodna istraživanja autora određivanja sadržaja vitamina C u plodovima određenih vrsta glogova (TAHIROVIĆ ET AL., 2012), sadržaji vitamina C u plodovima i cvjetovima vrsta C. monogyna i C. microphylla se odnose obrnuto.

Dobiveni rezultati jasno ukazuju da su cvjetovi ispitivanih vrsta glogova i hibrida dobar izvor vitamina C. Rezultati su značajni s aspekta evaluacije kvaliteta glogova i njihove primjene u farmaceutskoj industriji.

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01. 12. 2012.

How to Cite

Tahirović, A., Čopra – Janićijević, A. ., Bašić, N., Klepo, L. ., & Subašić, M. (2012). Određivanje vitamina C u cvjetovima nekih bosanskohercegovačkih vrsta roda Crataegus L. Radovi Šumarskog Fakulteta Univerziteta U Sarajevu, 42(2), 1–12. https://doi.org/10.54652/rsf.2012.v42.i2.119

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