Sadržaj fenola i antioksidacijski kapacitet lišća i grančica Viscum album sa različitih domaćina drveća
DOI:
https://doi.org/10.54652/rsf.2017.v47.i2.62Ključne riječi:
antioxidant activity, phenols, leaves, mistletoe, twigs, Viscum albumSažetak
UDK: 547.56:582.728.4
U ovoj studiji je izvršeno određivanje sadržaja fenolskih jedinjenja i antioksidacijskog kapaciteta ekstrakata listova i grančica imele (Viscum album ssp. album Beck.) spektrofotometrijskim metodama. Imela je prikupljana sa četiri domaćina: Crataegus monogyna, Malus domestica, Prunus cerasifera i Populus x canadensis. Određivanje ukupnih fenola, flavonoida, fenolskih kiselina i proantocijanidina vršeno je Folin–Ciocalteu metodom, AlCl3 metodom, Arnow reagensom i kiselinsko-butanolnom metodom. Antioksidacijski kapacitet ekstrakata ispitivan je DPPH, ABTS i FRAP metodama. Ukupni fenoli kretali su se u granicama 7,16-11,70 mg GAE/g s.u., flavonoidi 2,115-4,668 mg RE/ g s.u., fenolske kiseline 0,811-1.789 mg CAE/g s.u. i proantocijanidini 0,71-2,62 mg LCE/g s.u. Dobiveni sadržaj fenola i flavonoida veći je u listovima u odnosu na grančice. Listovi imele prikupljeni sa C. monogyna imali su najveći antioksidacijski kapacitet (67,28-81,72 µmol TE/g s.u.), a grančice imele prikupljene sa M. domestica (44,63–57.13 µmol TE/g s.u.). Uočena je visoka korelacija između sadržaja ukupnih fenola, fenolskih kiselina, proantocijanidina i antioksidacijske aktivnosti za listove, kao i ukupnih fenola i proantocijanidina za grančice imele.
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