Total phenolic content, antioxidant and antimicrobial activity of Silene sendtneri Boiss. (Caryophyllaceae)
DOI:
https://doi.org/10.54652/rsf.2022.v52.i1.367Abstract
Genus Silene (Caryophyllaceae) is very rich in secondary metabolites and has an antiviral, antimalarial, antitumor, antibacterial and antioxidant properties, but Balkan endemic Silene sendtneri (Sendtner's campion) is not analyzed from the aspect of phenolic composition and their biological activities. Evaluation of total phenolic contents (TPC) and total flavonoid contents (TFC), and antioxidant and antimicrobial activities of hydromethanolic extracts from inflorescences, stem, rhizome, and seeds of S. sendtneri, was done in this study for the first time. The TPC, TFC, and antioxidant activity (DPPH; 2,2-diphenyl-1-picrylhydrazyl) were determined by UV/VIS spectrophotometry. Antimicrobial activity was estimated against selected test microorganisms (Staphylococcus epidermididis, Staphylococcus aureus subsp. aureus, Salmonella abony, Escherichia coli, and Candida albicans) using a disc diffusion assay. The inflorescences had the highest (11.587 mg GAEg-1 DW) and rhizome the lowest TPC (2.017 mg GAEg-1 DW). The inflorescences extract exhibited the highest TFC (69.824 mg CEg-1 DW), while TFC was not detected in the rhizome extract. The stem’s extract had the highest antioxidant activity (IC50; 20.51%), while the rhizome had the lowest (61.89%). All extracts showed moderate antibacterial activity against Staphylococcus epidermididis and low activity against the three remaining tested organisms. Antifungal activity of inflorescence and rhizome extracts was moderate. Obtained results provide a basis for further investigations of various S. sendtneri extracts, which can be a potential natural antioxidant and antimicrobial agents
References
Ahmad, V.U., Ali, Z., Ali, M.S., & Zahid, M. (1998). Chemical constituents of Silene conoidea. Fitoterapia, 69(5), 406-408.
Alarcon, R., Ortiz, L.T., & Garcia, P. (2006). Nutrient and fatty acid composition of wild edible bladder campion populations [Silene vulgaris (Moench.) Garcke.]. International Journal of Food Science and Technology, 41, 1239-1242.
Aygun, R.B., Zengin, G., Yildiztugay, E., Jugreet, S., Yilmaz, M.A., & Mahomoodally, F.M. (2022). Chemical characterization, anti-oxidant and anti-enzymatic properties of extracts from two Silene species: A focus on different plant parts and extraction methods. Process Biochemistry, 116, 206-213.
Bajpai, V., Dung, N., Kwon, O., & Kang, S. (2008). Analysis and the potential applications of essential oil and leaf extracts of Silene armeria L. to control food spoilage and food-borne pathogens. European Food Research and Technology, 227, 1613-1620.
Bastola, K., Guragain, Y., Bhadriraju, V., & Vadlani, P. (2017). Evaluation of standards and interfering compounds in the determination of phenolics by folin-ciocalteu assay method for effective bioprocessing of biomass. American Journal of Analytical Chemistry, 8, 416-431.
Bauer, A.W., Kirby, W.M.M., Sherris, J.C., & Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 36, 493-496.
Bennett, R.N., & Wallsgrove, R.M. (2006). Secondary metabolites in plant defence mechanisms. New Phytologist, 127, 617-633.
Cai, Y., Luo, Q., Sun, M., & Corke, H. (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sciences, 74, 2157-2184.
Conforti, F., Marrelli, M., Carmela, C., Menichini, F., Valentina, P., Uzunov, D., Statti, G.A., Duez, P., & Menichini, F. (2011). Bioactive phytonutrients (omega fatty acids, tocopherols, polyphenols), in vitro inhibition of nitric oxide production and free radical scavenging activity of non-cultivated Mediterranean vegetables. Food Chemistry, 129, 1413-1419.
Dixon, R.A., & Paiva, N.L. (1995). Stress-induced phenylpropanoid metabolism. Plant Cell, 7, 1085-1097.
Đug, S., Muratović, E., Drešković, N., Boškailo, A., & Dudević, S. (2013). Crvena lista flore Federacije Bosne i Hercegovine. EU “Greenway”, Sarajevo, p.p. 161.
Eggens, F. (2006). Systematics in Sileneae (Caryophyllaceae) - Taxonomy and phylogenetic patterns. Acta Universitatis Upsaliensis, Digital Comprehensive Summaries of Uppsala, Dissertations from the Faculty of Science and Technology, p.p. 251.
Ertürk, O., Kati, H., Yayli, N., & Demirbag, Z. (2006). Antimicrobial properties of Silene multifida (Adams) Rohrb. plant extracts. Turkish Journal of Biology, 30, 17-21.
Fabricant, D.S., & Farnsworth, N.R. (2001). The value of plants used in traditional medicine for drug discovery. Environmental Health Perspectives, 109, 69-72.
Filippidis, A., Papastergios, G., Kantiranis, N., Michailidis, K., Chatzikirkou, A., & Katirtzoglou, K. (2012). The species of Silene compacta Fischer as indicator of zinc, iron and copper mineralization. Chemie der Erde, 72, 71-76.
Golovko, V., & Bushneva, O. (2007). Stabilizing effect of Silene pectin polysaccharide on electrical activity of the sinoatrial area in frog heart. Bulletin of Experimental Biology and Medicine, 143, 284-286.
Grlić, Lj. (1990). Enciklopedija samoniklog jestivog bilja. August Cesarec, Zagreb. p.p. 117.
Hartmann Th. 2007. From waste products to ecochemicals: Fifty years research of plant secondary metabolism. Phytochemistry, 68, 2831-2846.
Hasanuzzaman, M., Bhuyan, M., Zulfiqar, F., Raza, A., Mohsin, S.M., Mahmud, J.A., Fujita, M., & Fotopoulos, V. (2020). Reactive oxygen species and antioxidant defense in plants under abiotic stress: Revisiting the crucial role of a universal defense regulator. Antioxidants (Basel, Switzerland), 9(8), 681.
Hossain, M.A., & Shah, M.D. (2015). A study on the total phenols content and antioxidant activity of essential oil and different solvent extracts of endemic plant Merremia borneensis. Arabian Journal of Chemistry, 8(1), 66-71.
Jakimiuk, K., Wink, M., & Tomczyk, M. (2022). Flavonoids of the Caryophyllaceae. Phytochemistry Reviews, 21, 179-218.
Kabera, J.N., Semana, E., Mussa, A.R., & He, X. (2014). Plant secondary metabolites: Biosynthesis, classification, function and pharmacological properties. Journal of Pharmacy and Pharmacology, 2, 377-392.
Kähkönen, M.P., Hopia, A.I., Vuorela, H.J., Rauha, J.P., Pihlaja, K., Kujala, T.S., & Heinonen, M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agriculture and Food Chemistry, 47, 3954-3962.
Kandoudi, W., & Németh-Zámboriné, É. (2022). Stimulating secondary compound accumulation by elicitation: Is it a realistic tool in medicinal plants in vivo?. Phytochemistry Reviews. https://doi.org/10.1007/s11101-022-09822-3.
Karalija, E., Selović, A., Dahija, S., Demir, A., Samardžić, J., Vrobel, O., Ćavar Zeljković, S., & Parić, A. (2021). Use of seed priming to improve Cd accumulation and tolerance in Silene sendtneri, novel Cd hyper-accumulator. Ecotoxicology and Environmental Safety, 210, 111882.
Karamian, R., & Ghasemlou, F. (2013). Screening of total phenol and flavonoid content, antioxidant and antibacterial activities of the methanolic extracts of three Silene species from Iran. International Journal of Agriculture and Crop Sciences, 5, 305-312.
Kaur, S., & Mondal, P. (2014). Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. Journal of Microbiology & Experimentation, 1(1), 00005.
Kliebenstein, J.D. (2013). Making new molecules – evolution of structures for novel metabolites in plants. Current Opinion in Plant Biology, 16, 112-117.
Kucukboyaci, N., Ozcelik, B., Adiguzel, N., & Goren, A. (2010). Fatty-acid compositions of Silene vulgaris and S. cserei subsp. aeoniopsis seeds and their antimicrobial activities. Chemistry of Natural Compounds, 46, 88-91.
Mamadalieva, N.Z., Zibareva, L.N., Lafont, R., Dainan, L., & Saatov, Z. (2004). Phytoecdysteroids from the Silene genus. Chemistry of Natural Compounds, 40(6), 574-578.
Mamadalieva, N.Z. (2012). Phytoecdysteroids from Silene plants: distribution, diversity and biological (antitumour, antibacterial and antioxidant) activities. Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas, 11(6), 474-497.
Mamadalieva, N.Z., Egamberdieva, D., & Tiezzi, A. (2013). In vitro biological activities of the components from Silene wallichiana. Medicinal and Aromatic Plant Science and Biotechnology, 7, 1-6.
Mamadalieva, N.Z., Lafont, R., & Wink, M. (2014). Diversity of secondary metabolites in the genus Silene L. (Caryophyllaceae) – structures, distribution, and biological properties. Diversity, 6, 415-499.
Mamadalieva, N.Z., Ul’chenko, N.T., Yuldasheva, N.K., Zhanibekov, A.A., Egamberdieva, D.R., & Glushenkova, A.I. (2010a). Neutral lipids and biological activity of the CHCl3 extract of the aerial part of Silene guntensis. Chemistry of Natural Compounds, 46, 621-622.
Mamadalieva, N.Z., Ul’chenko, N.T., Yuldasheva, N.K., Egamberdieva, D.R., Zhanibekov, A.A., Dzhukharova, M.K., & Glushenkova, A.I. (2010b). Fatty-acid composition and antibacterial activity of CHCl3 extracts of three plants of the genus Silene. Chemistry of Natural Compounds, 46, 95-96.
Marhold, K. (2011). Silene sendtneri. Caryophyllaceae. – In Euro+MedPlantbase - the information resource for Euro-Mediterranean plant diversity [accessed 07.07.2022.].
Meda, A., Lamien, C.E., Romito, M., Millogo, J., & Nacoulma, O.G. (2005). Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan Honey, as well as their radical scavenging activity. Food Chemistry, 91(3), 571-577.
Medda, S., Fadda, A., & Mulas, M. (2022). Influence of climate change on metabolism and biological characteristics in perennial woody fruit crops in the Mediterranean environment. Horticulturae, 8(4), 273.
Mihajilov-Krstev, T., Zlatković, B., Ilić, M., Stankov-Jovanović, V., & Mitić, V. (2015). Antimicrobial and antioxidant potential of wild growing Silene baccifera (L.) Roth. (Caryophyllaceae) fruits juice. Biologica Nyssana, 6(2), 55-58.
Mišić, LJ., & Lakušić, R. (1990). Livadske biljke. 1st ed. IP Svjetlost, Zavod za udžbenike i nastavna sredstva, Sarajevo – Zavod za udžbenike i nastavna sredstva, Beograd. p.p. 24.
Morrisey, J.P., & Osbourn, A. (1999). Fungal resistance to plant antibiotics as a mechanism of pathogenesis. Microbiology and Molecular Biology Reviews, 63, 708-724.
Mouffouk, C., Mouffouk, S., Dekkiche, S., Hambaba, L., & Mouffouk, S. (2019). Antioxidant and antibacterial activities of the species Silene inflata Sm. PSM Biological Research [Internet], 4(2), 74-86.
Nadgórska-Socha, A., Kandziora-Ciupa, M., Ciepal, R., & Walasek, K. (2011). Effects of Zn, Cd, Pb on physiological response of Silene vulgaris plants from selected populations. Polish Journal of Environmental Studies, 20(3), 599-604.
Ordoñez, A., Gomez, J., Vattuone, M., & Isla, M. (2006). Antioxidant activities of Sechium edule (Jacq.) Swart extracts. Food Chemistry, 97, 452-458.
Oreopoulou, V. (2003). Extraction of natural antioxidants. In Tzia, C., Liadakis, G. (Ed.), Extraction optimization in food engineering, Marcel Dekker, New York.
Ribera, A., & Zúñiga, G.E. (2012). Induced plant secondary metabolites for phytopatogenic fungi control: a review. Journal of Soil Science and Plant Nutrition, 12(4), 893-911.
Royal Botanic Gardens, Kew, https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:331962-2, (30/06/2022).
Santos-Sánchez, N.F., Salas-Coronado, R., Villanueva-Cañongo, C., & Hernández-Carlos, B. (2019). Antioxidant compounds and their antioxidant mechanism. In Shalaby, E., (Ed.), Antioxidants [Internet]. London: IntechOpen, https://www.intechopen.com/chapters/66259, (06/07/2022).
Selitrennikoff, C.P. (2001). Antifungal proteins. Applied and Environmental Microbiology, 67, 2883-2894.
Shahidi, F., Janitha, P.K., & Wanasundara, P.D. (1992). Phenolic antioxidants. Critical Reviews in Food Science and Nutrition, 32(1), 67-103.
Sharma, A., Shahzad, B., Rehman, A., Bhardwaj, R., Landi, M., & Zheng, B. (2019). Response of phenylpropanoid pathway and the role of polyphenols in plants under abiotic stress. Molecules, 24(13), 2452.
Šilić, Č. (1988). Endemične biljke. 2nd ed, IP „Svjetlost“; Zavod za udžbenike i nastavna sredstva, Sarajevo; Zavod za udžbenike i nastavna sredstva, Beograd. p.p. 31.
Singelton, V.L., Orthofer, R., & Lamuela-Raventos, R.M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178.
Spiridon, I., Bodirlau, R., & Teaca, C.-A. (2011). Total phenolic content and antioxidant activity of plants used in traditional Romanian herbal medicine. Central European Journal of Biology, 6(3), 388-396.
Taskin, T., & Bitis, L. (2013). Antioxidant activity of Silene alba subsp. divaricata and Stellaria media subsp. media from Caryophyllaceae. Spatula DD, 3(1), 1-5.
Verpoorte, R. (2000). Pharmacognosy in the new millenium: leadfinding and biotechnology. Journal of Pharmacy and Pharmacology, 52, 253-262.
Webster, D., Taschereau, P., Belland, R.J., Sand, C., & Rennie, R.P. (2008). Antifungal activity of medicinal plant extracts. Preliminary screening studies. Journal of Ethnopharmacology, 115, 140-146.
Wink, M. (2008). Plant secondary metabolism: Diversity, function and its evolution. Natural Product Communications, 3(8), 1205-1216.
Wolfe, K., Wu, X., & Liu, H.R. (2003). Antioxidant activity of apple peels. Journal of Agricultural and Food Chemistry, 51, 609-614.
Zheng, W., & Wang, S.Y. 2001. Antioxidant activity and phenolic compounds in selected herbs. Journal of Agriculture and Food Chemistry, 49, 5165-5170.
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Copyright (c) 2022 Fatima Pustahija, Sabina Rastoder, Rasim Duraković Duraković, Mirel Subašić, Neđad Bašić, Adisa Parić
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