PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF CRATAEGUS MONOGYNA L. FRUIT EXTRACTS
DOI:
https://doi.org/10.54652/rsf.2014.v44.i2.99Keywords:
Crataegus monogyna L., natural phenolic compounds, antioxidant activityAbstract
UDK: 547.56:582.711.714
582.711.714:581.47
The aim of this work was to determine the content of total polyphenols, total flavonoids, total monomeric anthocyanins, total proanthocyanidins, and antioxidant activity of Crataegus monogyna L. fruit in water, hydroalcohol and alcohol extracts. Phenolic content and antioxidant assay of the different fruit extracts was determined using spectrophotometric methods. Obtained results indicated that the content of total polyphenols in the investigated extracts varied from 2.01 to 4.60 mg GAE g-1 of fresh hawthorn fruit. The content of flavonoids ranged from 0.254 to 0.595 mg RUE g-1 fresh fruit extracts. Total monomeric anthocyanins varied from 0.004 to 0.132 mg cyanidin-3-glucoside g-1 of fresh fruit and total proanthocyanidins varied from 0.187 to 1.168 mg cyanidin chloride g-1 fresh fruit. The best antioxidant activity was obtained for hawthorn extract with 80% methanol. A good correlation between antioxidant activity and total polyphenols (R² = 0.9473) and proanthocyanidins (R² = 0.7469) was observed.
References
BAHORUN, T., AUMJAND, E., RAMPHUL, H., RYEHA, M., LUXIMON-RAMMA, A., TROTIN, F. (2003): Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts. Nahrung, 47 (3): 191-8.
BAHORUN, T., TROTIN, F., POMMERY, J., VASSER, J., PINKAS, M. (1994): Antioxidant activities of Crataegus monogyna Extracts. Planta Medica, 60: 323-328.
BAHRI-SAHLOUL, R., AMMAR, S., GREC, S., HARZALLAH-SKHIRI, F. (2009 B): Chemical characterization of Crataegus azarolus L. fruit from 14 genotypes found in Tunisia. Journal of Horticulture Science of Biotechnology, 84, 23-28.
BARROS, L., CARVALHO, A.M., FERREIRA, I.C.F.R. (2010): Comparing the composition and bioactivity of Crataegus monogyna flowers and fruits used in folk medicine, Phytochemical Analysis. 22, 181–188.
BAŠIĆ, N. (2004): Morfološko-taksonomska istraživanja glogova (Crataegus L.) na području Bosne i Hercegovine. Magistarski rad. Sarajevo.
BECK, G. (1927): Flora Bosne, Hercegovine i oblasti Novoga Pazara. III. Choripetalae (Kaj): 169-172. Beograd-Sarajevo
BERNATONIENE, J., MASTEIKOVA, R., MAJIENE D., SAVICKAS, A., KEVELAITIS, E., BERNATONIENE, R., DVORAČKOVA, K., CIVINSKIENE, G., LEKAS, R., VITKEVIČIUS, K., PEČIURA, R. (2008): Free radical-scavenging activities of Crataegus monogyna extracts. Medicina (Kanaus), 44(9).
CAI, YZ., MEI, SUN, JIE XING (2006): Structure-radical scavenging from traditional Chinese medicinal plants. Life Science, 78(25):2872-88
CHANG, Q., ZUO, Z., HARRISON, F., CHOW, M.S.S., (2002): Hawthorns: An overview of chemical, pharmacological and clinical studies. Journal of Clinical Pharmacology. 42, 605-612.
CHRISTENSEN, K. I. (1992): Revision of Crataegus Sect. Crataegus and Notosect. Crataegineae (Rosaceae-Maloideae) in the old world. Systematic Botany Monographs, 35: 1-199.
CHRISTENSEN, K. I., JANJIĆ, N. (2006): Taxonomic notes on European taxa of Crataegus (Rosaceae). Nordic Journal of Botany, 24: 143-147.
THE COUNCIL OF EUROPE (2004): European Pharmacopoeia, fifth ed. Edqm, Strasbourg, pp. 1712-1715.
EDWARDS, JE., BROWN, PN., TALENT, N., DICKINSON, TA., SHIPLEY, PR. (2012): A review of the chemistry of the genus Crataegus. Phytochemistry, 79, 5-26.
EGEA, I., SANCEZ-BEL, P., ROMOJARO, F., PRETEL, MT. (2010): Six edible wild fruits as potential antioxidant additives or nutritional supplements. Plant Foods Human Nutrition 65:121-129.
FROEHLICHER, T., HENNEBELLE, T., MARTIN-NIZARD, F., CLEENEWERCK, P., HILBERT, JL., TROTIN, F. (2009): Phenolic profiles and antioxidative effects of hawthorn cell suspensions, fresh fruits and medicinal dried parts. Food Chemistry, 115, 897- 903.
FUKAREK, P. (1974): Neke vrste drveća i grmlja koje su pogrešno navedene u Flori Bosne i Hercegovine i susjednih krajeva. ANU BiH-Radovi LIV, Odjeljenje prirodno-matematičkih nauka, 15: 45-60.
HAGERMAN, A.E. (2002): The Tannin Handbook. Miami University Oxford.
HALVORESEN, B.L., HOLTE K., MYHRSTAD, M.C., BARIKMO, I., HVATUM, E., REMBERG, S.F. ET AL. (2002): A systematic screening of total antioxidants in dietary plants. Journal of Nutrition, 132(3), 461-71.
JANJIĆ, N. (1998): Neki zanimljivi dendrološki nalazi iz sarajevskog područja. Radovi Šumarskog Fakulteta Univerziteta u Sarajevu, 28(1): 85-103.
JANJIĆ, N. (2002): Nova kombinacija u lepezolisnog ili krivočašičnog gloga, Crataegus rhipidophylla Gand. (Rosaceae). Radovi Šumarskog Fakulteta Univerziteta u Sarajevu, 32(1): 1-7.
JUNG, S.J., KIM, D.H., HONG, YH (2007): Flavonoids from the flower of Rhododendron yedoense var. Poukhanense and their antioxidant activity. Arch Pharm Res, 30(2), 146-50.
KATALINIC, V., MILOS, M., KULISIC, T., JUKIC, M. (2006): Screening of 70 medicinal plant extracts for antioxidant capacity and phenols. Food Chemistry, 94, 550-557.
KIM, S.H., KANG, K.W., KIM, K.W., KIM, N.D. (2000): Procyanidins in Crataegus extract evoke endothelium-dependent vasorelaxation in rat aorta. Life Science, 67:121-131.
LEE, J., DURST, RW., WROLSTAD, E. (2005): Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by pH differential method: Collaborative study. Journal of AOAC International, 88(5), 1269-1278.
LJUBUNCIC, P., PORTNAYA, I., COGAN, U., AZAIZEH, H., BOMZON, A. (2005): Antioxidant activity of Crataegus aronia aqueous extract used in traditional Arab medicine in Israel. Journal of ethno pharmacology. 101, 153-161.
MALÝ, K. (1919): Prilozi za floru Bosne i Hercegovine 5 i 6. Glasnik Zemaljskog Muzeja BiH, Sarajevo, 31: 61-92.
MALÝ, K.. (1940): Notizen zur Flora von Bosnien-Herzegovina. Glasnik Zemaljskog Muzeja BiH, Sarajevo, 52: 21-46.
MILIAUSKAUS, G., VAN BEEK, TA., DE WAARD, P. (2005): Identification of radical scavenging compounds in Rhaponticum carthamoides by means of LC-DAD-SPE-NMR. Journal of Natural Products; 68:68-72.
MRAIHI, F., JOURNI, M., CHERIF, J.K., SOKMEN, M., SOKMEN, A., TRABELSI-AYADI, M. (2013): Phenolic content and antioxidant potential of Crataegus fruits grown in Tunisia as determined by DPPH, FRAP, and β-carotene/linoleic acid assay. Journal of chemistry, volume 2013, 1-6.
PIETTA, P.-G. (2000): Flavonoids as antioxidants. Journal of Natural Products. 63, 1035.
PINELO, M., RUBILAR, M., JEREZ, M., SINEIRO, J., NUNEZ, MJ. (2005): Effect of solvent, temperature, and solvent-to-solvent ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace. Journal of Agricultural Food Chemistry, 53, 2111-2117.
QUETTIER-DELEU, C., VOISELLE, G., FRUCHART, J.C., DURIEZ, P., TEISSIER, E., BAILLEUL, F., VASSEUR, J., TROTIN, F. (2003): Hawthorn extracts inhibit LDL oxidation. Pharmazie, 58, 577-81.
QUETTIER, D.C., GRESSIER, B., VASSEUR, J., DINE, T., BRUNET, C., LUYCK, M.C., CAYIN, J.C., BAILLEUL, F., TROTIN, F. (2000): Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of Ethnopharmacology, 118, 418-428.
SANCEZ-MORENO, C., LARRAURI, J.A., SAURA-CALIXTO, F. (1998): A procedure to measure the antiradical efficiency of polyphenols. Journal of Science Food Agriculture, 76, 270-276.
SINGLETON, V.L., ORTHOFER, R., LAMUELA-RAVENTOS, R.M. (1974): Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin_Ciocalteu reagent. Methods of Enzymology, 229, 152-178.
SKRGET, M.P., KOTNIK, M., HADOLIN, A.R., HRAS, A.R., SIMONIĆ, M., KNEZ, Z. (2005): Phenols, proanthocyanidins, flavons and flavonols in some plant materials and their antioxidant activities. Food Chemistry, 89:191-198.
SPIGNO, G., TRAMELL,I L., DE FAVERI, D.M., (2007): Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. Journal of Food Engineering. 81, 200-208.
STRATIL, P., KLEJDUS, B., KUBAN, V. (2007): Determination of phenolic compounds and their antioxidant activity in fruits and cereals. Talanta, 71, 17411-1751.
TADIC, M.V., DOBRIC, S., MARKOVIC, M.G., ĐORDEVIC, M.S., ARSIC, A.L. (2008): Anti-inflammatory, gastroprotective, free-radical-scavenging, and antimicrobial activities of hawthorn berries ethanol extract. Journal of Agricultural Food Chemistry, 56, 7700-7709.
WANG, M., SIMON, J.E., AVILES, I.F., HE K., ZHENG, Q.Y., TADMOR, Y. (2003): Analysis of antioxidative phenolic compounds in artichoke (Cynara scolymus L). Journal of Agricultural Food Chemistry, 5(3), 601-8.
ZHANG, Z., CHANG, Q., ZHU, M., HUANG, Y., HO, W.K.K., CHEN, Z.-Y. (2001): Characterization of antioxidants present in hawthorn fruits. Journal of Nutritional Biochemistry, 12, 144.
ZHOU, K. AND YU, L. (2006): Total phenolic contents and antioxidant properties of commonly consumed vegetables grown in Colorado. LWT-Food Science Technology, 39:1155-1162.
