PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF CRATAEGUS MONOGYNA JACQ. AND CRATAEGUS MACROCARPA HEGETSCHW. LEAVES AND FRUITS EXTRACTS

Authors

  • Azra Tahirović Faculty of Forestry University of Sarajevo
  • Neđad Bašić Faculty of Forestry University of Sarajevo

DOI:

https://doi.org/10.54652/rsf.2015.v45.i2.83

Keywords:

C. monogyna, C. x macrocarpa, phenols, antioxidant capacity

Abstract

UDK: 547.56:582.711.714

          582.711.714:581.45/.47

Phenolic contents of methanolic extracts prepared from leaves and fruits of Crataegus monogyna Jacq. and Crataegus macrocarpa Hegetschw. were determined. The antioxidant capacity was assessed by DPPH, ABTS and FRAP assay. The results showed that leaves were richer in the content of phenols (59.23 - 91.91 GAE g-1), flavonoids (2.38 - 4.08 mg QE g-1 and 5.24 - 8.9 mg RE g-1) , phenolic acids (33.40 - 68.98 CAE g-1) and proanthocyanidins (26.15 - 48.60 CE g-1) while in fruits anthocyanins dominateted (0.43 - 0.80 CG g-1). Leaves also had higher antioxidant capacity than fruits for both species. Generelly, C. monogyna fruits had higher content of anthocyanins. Total phenols, phenolic acids and proanthocyanidins were highly corelated with DPPH (r2 = 0.8703 - 0.9618), ABTS (r2 = 0.7833 - 0.9443) and FRAP (r2 = 0.903 - 0.9695) assay. The results suggests that these compounds were the major contributors to the antioxidant capacity in leaves and fruits extracts of both species.

Higher contents of bioactive compounds and higher antioxidant capacity were determined for C. x macrocarpa samples. Therefore, C. x macrocarpa leaves and fruits are valuble source of antioxidant polyphenols with high potential for use in preparation of different natural health products.

References

ABDENNACER, B., KARIM, M., YASSINE, M. NESRINE, R., MOUNA, D., MOHAMED, B. (2015): Determination of phytochemicals and antioxidant activity of methanol extracts obtained from the fruits and leaves of Tuniisian Lycium intricatum Boiss. Food Chemistry, 174, 577-584.

BAHORUN, T., TROTIN, F., POMMERY, J., VASSER, J., PINKAS, M. (1994): Antioxidant activities of Crataegus monogyna Extracts. Planta Medica, 60: 323-328.

BAHRI-SAHLOUL, R., AMMAR, S., GREC, S., HARZALLAH-SKHIRI, F. (2009B): Chemical characterization of Crataegus azarolus L. fruit from 14 genotypes found in Tunisia. Journal of Horticulture Science of Biotechnology, 84, 23-28.

BARROS, L., CARVALHO, A.M., FERREIRA, I.C.F.R. (2010): Comparing the composition and bioactivity of Crataegus monogyna flowers and fruits used in folk medicine, Phytochemical Analysis. 22, 181–188.

BAŠIĆ, N. (2004): Morfološko-taksonomska istraživanja glogova (Crataegus L.) na području Bosne i Hercegovine. Magistarski rad. Sarajevo.

BECK, G. (1927): Flora Bosne, Hercegovine i oblasti Novoga Pazara. III. Choripetalae (Kaj): 169-172. Beograd-Sarajevo

BENZİE I.F. AND STRAİN J.J. (1999): The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Analytical Biochemistry, 239(1) 70-6.

BERKER, K.I.; GÜÇLÜ, K.; TOR, I.; APAK, R. (2007): Comparative Evaluation of Fe(III) Reducing PowerBased Antioxidant Capacity Assays in the Presence of Phenanthroline, Batho-phenanthroline, Tripyridyltriazine (FRAP), and Ferricyanide Reagents, Talanta, 72, 1157-1165.

BERNATONIENE, J., MASTEIKOVA, R., MAJIENE D., SAVICKAS, A., KEVELAITIS, E., BERNATONIENE, R., DVORAČKOVA, K., CIVINSKIENE, G., LEKAS, R., VITKEVIČIUS, K., PEČIURA, R. (2008): Free radical-scavenging activities of Crataegus monogyna extracts. Medicina (Kanaus), 44(9).

BRAND-WİLLİAMS W, CUVELİER ME, BERSET C. (1995): Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technology, 28:25-30.

CHANG, Q., ZUO, Z., HARRISON, F., CHOW, M.S.S. (2002): Hawthorns: An overview of chemical, pharmacological and clinical studies. Journal of Clinical Pharmacology. 42, 605-612.

CHEN, J.H., AND HO, C.T. (1997): Antioxidant activity of caffeic acid and its related hydroxycinammic acid compounds. Journal of Agricultural Food Chemistry. 45, 2374-2378.

CHRIST, B. AND MULLER, K.H. (1960): Determination of the amount of flavonol derivates in drugs. Archiv der pharmazie, 293, 1033-1042.

CHRISTENSEN, K. I. AND JANJIĆ, N. (2006): Taxonomic notes on European taxa of Crataegus (Rosaceae). Nordic Journal of Botany, 24: 143-147.

EDWARDS, J.E., BROWN, P.N., TALENT, N., DİCKİNSON, T.A., SHİPLEY, P.R. (2012): A review of the chemistry of the genus Crataegus. Phytochemistry, 79, 5-26.

EGEA, I., SANCEZ-BEL, P., ROMOJARO, F., PRETEL, MT. (2010): Six edible wild fruits as potential antioxidant additives or nutritional supplements. Plant Foods Human Nutrition 65:121-129.

FUKAREK, P. (1974): Neke vrste drveća i grmlja koje su pogrešno navedene u Flori Bosne i Hercegovine i susjednih krajeva. ANU BiH-Radovi LIV, Odjeljenje prirodno-matematičkih nauka, 15: 45-60.

GAWLIC-DZIKI, U. (2012): Dietary spices as natural effectors of lipoxygenase, xanthine oxidase, peroxidase and antioxidant agents. LTW-Food Science and Technology, 47, 138-146.

HAGERMAN, A.E. (2002): The Tannin Handbook. Miami University Oxford.

HARNAFI, H. AND AMRANI, S. (2008). Spectrophotometric methods for determination of plant polyphenols content and their antioxidant activity assessment: An Overview, Pharmacognosy Reviews, 2(3), 20-22.

HRABĔTOVÁ-UHROVÁ A. (1969): Hloh (Crataegus L.) v Československu. Preslia, (Praha). 41: 162-182.

JANJIĆ, N. (1998): Neki zanimljivi dendrološki nalazi iz sarajevskog područja. Radovi Šumarskog Fakulteta Univerziteta u Sarajevu, 28(1): 85-103.

JUNG, S.J., KIM, D.H., HONG, Y.H. (2007): Flavonoids from the flower of Rhododendron yedonese var. poukhanense and their antioxidant activities. Archives of Pharmacal Research, 30(2), 146:50.

KIM, S.H., KANG, K.W., KIM, K. W., KIM, N.D. (2000): Procyanidins in Crataegus extract evoke endothelium-dependent vasorelaxation in rat aorta. Life Science, 67:121-131.

LEE, J., DURST, RW., WROLSTAD, E. (2005): Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by pH differential method: Collaborative study. Journal of AOAC International, 88(5), 1269-1278.

LIU, R.H., YU, B.Y., QIU, S.X., ZHENG, D. (2005): Comparative analysis of eight major polyphenolic compounds in leaves of Crataegus L. by HPLC. Chinese Journal of Natural Medicine, 3, 162-167.

MALÝ, K. (1919): Prilozi za floru Bosne i Hercegovine 5 i 6. Glasnik Zemaljskog Muzeja BiH, Sarajevo, 31: 61-92.

MALÝ, K. (1940): Notizen zur Flora von Bosnien-Herzegovina. Glasnik Zemaljskog Muzeja BiH, Sarajevo, 52: 21-46.

MANAH, C., SCALBERT, A, MORAND, C., REMESY, C., JIMENEZ, L. (2004): Polyphenols: food sources and bioavailability, The American Journal of Clinical Nutrition, 79:727-747.

MANDEL, S., AMIT, T., REZNICHENKO, L., WEINREB, O., YOUDIM, M-B. (2006): Green tea catechins as a brain permeable, natural iron chelators-antioxidants for the treatment of neurodegenerative disorders. Molecular Nutrition & Food Research, 50, 229-34

MRAIHI, F., JOURNI, M., CHERIF, JK. SOKMEN, M., SOKMEN, A., TRABELSI-AYADI, M. (2013): Phenolic content and antioxidant potential of Crataegus fruits grown in Tunisia as determined by DPPH, FRAP, and β-carotene/linoleic acid assay. Journal of Chemistry, volume 2013, 1-6.

NAZ, S., SIDDIQI, R., AHMAD, S., RASOOL, S.A., SAYEED, S.A., (2007): Antibacterial activity directed isolation of compounds from Punica granatum. Journal of Food Science, 72, 341-345.

OLTHOF, M.R., HOLMAN, P.C.H., KATAN, M.B. (2001): Chlorogenic acid and caffeic acid are absorbed in human. Journal of Nutrion, 131, 66-71.

OSAWA, T. (1994): Novel natural antioxidants for utilization in food and biological systems in Postharvest Biochemistry of Plant Food-Materials in the Tropics, I. Uritani, V.V. Garcia, and E.M. Mendoza, Eds., 241-251, Japan Scientific Societies Press.

ÖZCAN, M., HACISEFEROGULLARY, H., MARAKOGLU, T., ARSLAN, D. (2005). Hawthorn (Crataegus spp) fruit: Some physical and chemical properties. Journal of Food Engineering, 69, 409-413.

ÖZYÜREK, M., BENER, M., GÜÇLÜ, K., DÖNMEZ, A.A., SÜZGEÇ-SELÇUK, S., PIRILDAR, S., MERIÇLI, A.H., APAK, R. (2012): Evaluation of antioxidant activity of Crataegus species collected from different regions of Turkey. Records of Natural Products, 6:3, 262-277.

RE R, PELLEGRİNİ N, PROTEGGENTE A, PANNALA A, YANG M, RİCE-EVANS C.(1999): Antioxidant activity applying an improved ABTS radical cation decolorization assay.Free Radical Biology and Medicine, 26, (9-10), 1231-7.

RICE-EVANS, C.A., MILLER, N.J. (1997): Structure-antioxidant activity relationship of flavonoids and isoflavonoids. In: Ricde-Evans, C.A., packer, L. (Eds.), Flavonoids in Health and Disease. Marcel Dekker, New York.

RICE-EVANS, C.A., MILLER, N.J., BOLWELL, P.G., BRAMLEY, P.M., PRIDHAM, J.B. (1995): The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radical Research, 22 (4), 375-383.

RUIZ-RODRIGEZ, B., DE ANCOS, B. SANCEZ-MORENO, C., FERNANDEZ-RUIZ, V., SANCEZ-MATA, M., CAMARA, M., TARDIO, J. (2014). Wild blackthorn (Prunus spinosa L.) and hawthorn (Crataegus monogyna Jacq.) as valuable source of antioxidants. Fruits, 69, 61-73.

SEİFRİED, H. E., ANDERSON, D. E., FİSHER, E. I. AND MİLNER, J. A. (2007). A review of the interaction among dietary antioxidants and reactive oxygen species. The Journal of Nutritional Biochemistry, 18 (9): 567-579.

SINGLETON, V.L., ORTHOFER, R., LAMUELA-RAVENTOS, R.M. (1974): Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods of Enzymology, 229, 152-178.

TAMPLE, N.J. (2000): Antioxidants and disease: more questions than answers. Nutrition Research, 20,449-459.

THAIPONG, K., BOONPRAKOB, U., CROSBY, K., CISNEROS-ZEVALLOS, L., BYRNE, D.H. (2006): Comparison of ABTS, DPPH, FRAP and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis, 19, 669-675.

YU, L., HALEY, S., PERRET, J., HARRIS, M., WILSON, J., QIAN, M. (2002): Free radical scavenging properties of wheat extracts. Journal of Agricultural Food Chemistry, 50:1619-1624.

ZHANG, Z., CHANG, Q., ZHU, M., HUANG, Y., HO, W.K.K., CHEN, Z.-Y. (2001): Characterization of antioxidants present in hawthorn fruits. Journal of Nutritional Biochemistry, 12, 144.

Downloads

Published

01. 12. 2015.

How to Cite

Tahirović, A., & Bašić, N. (2015). PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF CRATAEGUS MONOGYNA JACQ. AND CRATAEGUS MACROCARPA HEGETSCHW. LEAVES AND FRUITS EXTRACTS. Works of the Faculty of Forestry University of Sarajevo, 45(2), 37–51. https://doi.org/10.54652/rsf.2015.v45.i2.83

Most read articles by the same author(s)

1 2 3 > >>