DETERMINATION OF VITAMIN C IN FLOWERS OF SOME BOSNIAN CRATAEGUS L. SPECIES
DOI:
https://doi.org/10.54652/rsf.2012.v42.i2.119Keywords:
ascorbic acid, Crataegus, hawthorns, flowers, spectrophotometry, Vitamin CAbstract
UDK 582.711.714:577.164.2
543.42:577.164.2
Vitamin C or ascorbic acid content has been determinated by spectrophotometric method and titrimetric method in flowers of some Bosnian hawthorns (Crataegus L). species. Spectrophotometric method used in this study was based on the kinetic reaction between Vitamin C and methylene blue. Measurements were carried out at absorption maximum, λmax= 665 nm. We found that the lowest content of vitamin C was 617.07 mg/100 g of dry sample in flowers of the C. microphylla, and the highest level of Vitamin C was found in the C. monogyna (1104 mg/100 g of dry sample) flowers. Recoveries of the results obtained by the spectrophotometric method were 94 % - 100% with relative standard deviation (RSD) values from 4.5% – 6.7 %.
Obtained results shown that flowers of investigated Crataegus L. species are good source of vitamin C.
References
AMMON, H.P.T., HANDEL, M. (1981): Crataegus, toxicology and pharmacology. Parts I- III. Planta Medica, 43: 1050-20, 209-39, 313-22.
AOAC International, official methods of analysis, 18th ed., AOAC International, Arlington, VA, 2005.
ARAYA, S. P., MAHAJAN, M., JAIN, P. (1998): Photometric methods for the determination of vitamin C. Analytical Sciences, 14: 889-894.
ARAYA, S. P., MAHAJAN, M., JAIN, P. (2000): Non-spectrophotometric methods for the determination of Vitamin C. Analytica Chimica Acta, 417: 1-14.
BAHORUN, T., TROTIN, F., POMMERY, J., VASSER, J., PINKAS, M. (1994): Antioxidant activities of Crataegus monogyna Extracts. Planta Medica, 60: 323-328.
BAHORUN, T., AUMJAND, E., RAMPHUL, H., RYEHA, M., LUXIMON-RAMMA, A., TROTIN, F. (2003): Phenolic constituents and antioxidant capacities of Crataegus monogyna (Hawthorn) callus extracts. Nahrung, 47 (3): 191-8.
BAJAJ, K.L., AND KAUR, G. (1981): Spectrophotometric determination of L-ascorbic acid in vegetable and fruits. Analyst, 106(1258): 117-20.
BARROS, L., CARVALHO, A.M., FERREIRA, C.F.R. (2011): Comparing the composition of bioactivity Crataegus monogyna flowers and fruits used in folk medicine. Phytochemical analysis, 22(2), 181-8.
BAŠIĆ, N. (2004): Morfološko-taksonomska istraživanja glogova (Crataegus L.) na području Bosne i Hercegovine. Magistarski rad. Sarajevo.
BECK, G. (1927): Flora Bosne, Hercegovine i oblasti Novoga Pazara. III. Choripetalae (Kaj): 169-172. Beograd-Sarajevo.
Bushway, R. J., KING, J. M., PERKINS, B. M., Krishnan, M. (1988): High-performance liquid chromatographic determination of ascorbic acid in fruits, vegetables and juices. Journal of Liquid Chromatography, 11: 3415 - 3423.
CHRISTENSEN, K. I. (1992): Revision of Crataegus Sect. Crataegus and Notosect. Crataegineae (Rosaceae-Maloideae) in the old world. Systematic Botany Monographs, 35: 1-199.
CHRISTENSEN, K. I., JANJIĆ, N. (2006): Taxonomic notes on European taxa of Crataegus (Rosaceae). Nordic Journal of Botany, 24: 143-147.
CUI, T., NAKAMURA, K., TIAN, S., KAYAHARA, H., TIAN, Y. L. (2006): Polyphenolic content and physiological activities of Chinese hawthorn extracts. Bioscience Biotechnology and Biochemistry, 70 (12): 2948-56.
DAVEY, M. W., VAN MONTAGU, M., INZÉ, D., SANMARTIN, M., KANELLIS, A., SMIRNOFF, N., BENZIE, I. J. J., STRAIN, J. J., FAVELL, D., FLETCHER, J. (2000): Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing. Journal of the Science of Food and Agriculture, 80, 825-860.
EGEA, I., SANCHEZ-BEL, P., RAMOJARO, F., PRETEL, M. T. (2010): Six edible wild fruits as potential antioxidant additives or nutritional supplements. Plant Foods for Human Nutrition, 65:121-129.
EITENMILLER, R. R., YE L., LANDEN, W. O. (2008): Vitamin analysis for the health and food sciences. CRC Press, Taylor & Francis Group, UK, 231-274.
ENGLARD, S., SEIFTER, S. (1986): The biochemical functions of ascorbic acid. Annual Review of Nutrition, 6: 365-406.
FOYER, C.H., LELANDIAS, M., EDWARDS, E.A., MULLINEAUX, P. M. (1991): Current topics in plant physiology, 6, ASPP, Rockville, 131.
FREDERIK, R. AND KLENNER, M.D. (1971): Observation on the dose of vitamins and administration of ascorbic acid. Journal of Applied Nutrition, 23: 2-3
FUKAREK, P. (1974): Neke vrste drveća i grmlja koje su pogrešno navedene u Flori Bosne i Hercegovine i susjednih krajeva. ANU BiH-Radovi LIV, Odjeljenje prirodno-matematičkih nauka, 15: 45-60.
HANACK, T., BRUCKEL, M. H. (1983): The treatment of mild stable forms of angina pectoris using crategutt novo. Therapiewoche, 33: 4331-4333.
HANSEL, R., KELLER, K., RIMPLER, H., SCHNEIDER, G. (1992): Hagers Handbuch der Pharmazeutischen Praxis, Vol. 4, Drogen A-D, 5th ed., Springer, Berlin, Heidelberg: 1040-1062.
IGBAL, K, KHAN, A., KHATAK, M.A.K (2004): Biological significance of ascorbic acid (Vitamin C) in human health – A review. Pakistan Journal of Nutrition, 3(1): 5-13.
JANJIĆ, N. (1998): Neki zanimljivi dendrološki nalazi iz sarajevskog područja. Radovi Šumarskog Fakulteta Univerziteta u Sarajevu, 28(1): 85-103.
JANJIĆ, N. (2002): Nova kombinacija u lepezolisnog ili krivočašičnog gloga, Crataegus rhipidophylla Gand. (Rosaceae). Radovi Šumarskog Fakulteta Univerziteta u Sarajevu, 32(1): 1-7.
KLEIN, B.P., AND PERRY, A.K. (1982): Ascorbic acid and vitamin A activity in selected vegetables from different geographical areas of the United States. Journal of Food Science, 47: 941-945.
MALÝ, K. (1919): Prilozi za floru Bosne i Hercegovine 5 i 6. Glasnik Zemaljskog Muzeja BiH, Sarajevo, 31: 61-92.
MALÝ, K. (1940): Notizen zur Flora von Bosnien-Herzegovina. Glasnik Zemaljskog Muzeja BiH, Sarajevo, 52: 21-46.
MARCUS, R. AND COULSTON, A.M. (2001): The Vitamins: In: Goodman and Gilmans The Pharmacological Basis of Therapeutics. 10th ed., Hardman J.G. and Limbirb LE (eds), Mcgraw-Hill Co., USA, pp: 1767-1771.
MOWRY, S., ORGEN, P. J. (1999): Kinetics of methylene blue reduction by ascorbic acid, Journal of Chemical Education, 76(7): 970-974.
OGULNESI, M., OKIEI, W., AZEEZ, L., OBAKACHI, V., OSUNSANMI, M., NKENCHOR, G.
(2010): Vitamin C contents of tropical vegetables and foods determined by voltammetric and titrimetric methods and their relevance to the medicinal uses of the plants. International Journal of Electrochemical Science, 5: 105-115.
PEREZ-RUIZ, T., MARTINEZ-LOZANO, K., TOMAS, V., PENOL, J. (2001): Fluorimetric
determination of total ascorbic acid by a stopped flow mixing technique. Analyst, 126, 1436-1439.
RASANU, N., MAGEARU, V., MATEI, N., SOCEANU, A. (2005): Determination of Vitamin C in different stages of fruits growing. Analele Universitatatii din Bucuresti- Chimie, Annual XIV, Vol. 1-11: 167-172.
SVEDSTROM, U., VUORELA, U.H., KOSTIAINEN, R., LAAKSO, I., HILTUNEN, R. (2006): Fractionation of polyphenols in hawthorn into polymeric procyanidins, phenolic acids and flavonoids prior to high-performance liquid chromatographic analysis. Journal of Chromatography, 1112: 103-111.
SZETO, Y.T., TOMLINSON, B., BENZIE, I.F. (2002): Total antioxidant and ascorbic acid content of fresh fruits and vegetables: implications for dietary planning and food preservation. The British Journal of Nutrition, 87 (1): 55-9.
TAHIROVIĆ, A., ČOPRA-JANIĆIJEVIĆ, A., BAŠIĆ, N., VIDIC, D., KLEPO L., DELIĆ, D. (2012): Determination of Vitamin C in some Bosnian Crataegus L. species by spectrophotometric method. Works of Faculty of Forestry University of Sarajevo, 42(1): 43-55.
URBONAVICIUTE, A., JAKSTAS, V., KORNISOVA, O., JANULIS, V., MARUSKA, A. (2006): Capillary electrophoretic analysis of flavonoids in single-styled hawthorn (Crataegus monogyna Jacq.) ethanolic extracts. Journal of Chromatography, 1112: 339-344.
VEVERIS, M., KOCH, E., CHATTERJEE S.S. (2004): Crataegus special extract WS®1442 improves cardiac function and reduce infarct size in a rat model of prolonged coronary ischemia and reperfusion. Life Science, 74: 1945-1955.
WHO/FAO (2004): Vitamin and mineral requirements in human nutrition. Second edition, World Health Organisation and Food and Agricultural Organisation of the United Nations, Sung-Fung, China, Chapter 7, 130-139.
ZAPIE JAN, G. (2001). Clinical efficacy of Crataegus extracts WS®1442 in congestive heart failure NYHA class II. Phytomedicine, 8: 262-266.
ZENG, W., MARTINUZZI, F., MAC GREGOR, A. (2005): Development and application of a novel UV method for the analysis of ascorbic acid. Journal of Pharmaceutical and Biomedicine Analysis. 36(5): 1107-1111.